| CatalogCode: | NSB458 |
| ProductName: | MEK1 Antibody |
| Product Description: | Rabbit Polyclonal anti-MEK1 [Thr292] |
| Clonality: | Polyclonal |
| Immunogen: | The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human MEK 1 that contains threonine 292. |
| CrossReactivity: | Cross-reacts with Human, Mouse and Rat.Not yet tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: Use at a concentration of 0.1 - 1 ug/ml. Predicted molecular weight: 50 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Control: | NIH3T3 cells +/- PDGF. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | Preservative: 0.05% Sodium AzideConstituents: PBS, 1mg/ml BSA (IgG, protease free). pH 7.3 |
| ListPrice: | 325 |
| AppSummary: | WB |
| SpeciesSummary: | Hu, Mu, Rt |
| ALTnames: | anti-MEK1 antibody |
| ProteinTarget: | MEK1 phospho T292 |
| PackageSize: | 0.1 ml |
| GeneralRef: | General / background references:Sharma P et al. Phosphorylation of MEK1 by cdk5/p35 down-regulates the mitogen-activated protein kinase pathway. J Biol Chem 277:528-34 (2002). Lin CC et al. Thrombin-stimulated cell proliferation mediated through activation of Ras/Raf/MEK/MAPK pathway in canine cultured tracheal smooth muscle cells. Cell Signal 14:265-75 (2002). Jin K et al. MEK and ERK protect hypoxic cortical neurons via phosphorylation of Bad. J Neurochem 80:119-25 (2002). Takahashi H et al. Expression of human cystatin A by keratinocytes is positively regulated via the Ras/MEKK1/MKK7/JNK signal transduction pathway but negatively regulated via the Ras/Raf-1/MEK1/ERK pathway. J Biol Chem 276:36632-8 (2001). Xu B et al. The N-terminal ERK-binding site of MEK1 is required for efficient feedback phosphorylation by ERK2 in vitro and ERK activation in vivo. J Biol Chem 274:34029-35 (1999). |
| Phosphorylated: | Thr292 |