| Catalog Code: | NSB431 |
| Product Type: | Primary Antibody |
| Product Name: | BimEL Antibody |
| List Price: | 310 |
| Clonality: | Polyclonal |
| Gene ID: | 10018 |
| Host: | Rabbit |
| Product Description: | Rabbit Polyclonal anti-BimEL |
| Cross Reactivity: | Rat BimEL. This antibody is observed to react with human BimEL when phosphorylated at serine 69. Mouse BimEL (100% homologous) has not been tested, but is expected to react. |
| Species Summary: | Hu, Rt |
| Background: | Bim (bcl-2-interacting mediator of cell death) is a proapoptotic member of the Bcl-2 family that shares only the BH3 domain with this family. There are three isoforms of Bim: BimEL, BimL, and BimS. Bim is involved in regulating the intrinsic mitochondrial |
| Immunogen: | The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of rat BimEL that contains serine 65 (serine 69 in the human sequence). The sequence is conserved in human and mouse. |
| Packaging: | 0.1 ml of epitope-specific affinity purified rabbit antisera in PBS(without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA (IgG, protease free) as a carrier. Contains 0.05% sodium azide (Caution: sodium azide is a poisonous and hazardo |
| Package Size: | 0.1 ml |
| Uses: | This antibody has been used in Western blotting. For Western blotting applications, we recommend using the antibody at a 1:1000 dilution. *The optimal antibody concentration should be determined empirically for each specific application. |
| Application Summary: | WB |
| General References: | 1. Yip, K.W., et al. (2004) Potential utility of bim(s) as a novel apoptotic therapeutic molecule. Mol. Ther. 10(3):533-544. 2. Ley, R. et al. (2004) Extracellular signal-regulated kinases 1/2 are serum-stimulated Bim(EL) kinases that bind to the BH3-onl |
| Notes Main: | Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Bim. |