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| Catalog Code: | NSB422 |
| Product Name: | JAK1 Phosphospecific Antibody |
| Product Description: | Rabbit Polyclonal anti-JAK1 [Tyr1022/Tyr1023] |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Immunogen: | Synthetic phospho peptide (Human). The sequence is conserved in mouse and pig. |
| Cross Reactivity: | Cross-reacts with Human and Mouse. Does not cross-react with Rat. Not yet tested in other species. |
| Packaging: | 0.1 ml Immunogen affinity purified Rabbit antisera. |
| Uses: | This antibody can be sued in Western Blot where a 130kDa band is seen. Not tested in other applications.Recommended Starting Dilutions*:WB: 1/1000*Optimal dilutions should be determined by the end user. |
| Localization: | wholly intracellular, possibly membrane associated |
| Background: | Janus Kinase 1 (JAK 1) is a 130 kDa tyrosine kinase involved in cytoplasmic signal transduction. Ligand binding to a variety of cell surface receptors (e.g., cytokine, growth factor, GPCRs) leads to an association of those receptors with JAK proteins, which are then activated via phosphorylation on tyrosines 1022 and 1023 in the kinase activation loop. Activated JAK proteins phosphorylate and activate STAT (signal transducers and activators of transcription) proteins, which then dimerize and translocate to the nucleus. Once in the nucleus, STAT proteins bind to DNA and modify the transcription of various genes, which can lead to various responses such as cell proliferation, cell survival and differentiation. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | Dulbeccos PBS containing 50% glycerol with 1.0 mg/mL BSA and 0.05% sodium azide |
| Application Summary: | Western Blot |
| Species Summary: | Hu, Mu, |
| Alternate Names: | anti-JAK1A antibody, anti-Tyrosine protein kinase antibody, anti-JAK-1 antibody, anti-JAK 1 antibody, anti-Janus Kinase 1 antibody |
| Package Size: | 0.1 ml |
| Phosphorylated: | Tyr1022/Tyr1023 |
| Product References: | Fukada T & Tonks NK Identification of YB-1 as a regulator of PTP1B expression: implications for regulation of insulin and cytokine signaling. EMBO J 22:479-93 (2003). Booz GW et al. Cytokine G-protein signaling crosstalk in cardiomyocytes: attenuation of Jak-STAT activation by endothelin-1. Mol Cell Biochem 240:39-46 (2002). General / background references:Ju H et al. Bradykinin activates the Janus-activated kinase/signal transducers and activators of transcription (JAK/STAT) pathway in vascular endothelial cells: localization of JAK/STAT signalling proteins in plasmalemmal caveolae. Biochem J 351:257-64 (2000). Liu KD et al. Janus kinases in interleukin-2-mediated signaling: JAK1 and JAK3 are differentially regulated by tyrosine phosphorylation. Curr Biol 7:817-26 (1997). Ihle JN STATs: signal transducers and activators of transcription. Cell 84:331-4 (1996). |
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