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| Catalog Code: | NSB390 |
| Product Name: | STAT5 Phosphospecific Antibody |
| Product Description: | Rabbit Polyclonal anti-STAT5 [Tyr694] |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Immunogen: | Synthetic peptide (Human). |
| Specificity: | NSB 390 recognises phosphorylated STAT5. |
| Cross Reactivity: | Cross-reacts with Mouse STAT5. Human STAT5a, rat STAT5a, human STAT5b (100% homologous), and mouse and rat STAT5b (92% homologous)Not yet tested in other species. |
| Packaging: | 0.1 ml Immunogen affinity purified Rabbit antisera. |
| Uses: | WB: Use at a dilution of 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 90.6 kDa).Can be blocked with Phospho and non phospho peptide pair for STAT5a [pY694]Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Nuclear |
| Background: | STATs, such as STAT5, are proteins that serve the dual function of signal transducers and activators of transcription in cells exposed to signaling polypeptides. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. STAT5 is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. STAT5a and STAT5b knockout mice show important roles in prolactin-mediated mammary gland development and growth hormone-mediated induction of sexual dimorphism, respectively. Abnormal activity of certain STAT family members, particularly STAT3 and STAT5, is associated with a wide variety of human malignancies, including hematologic, breast, head and neck, and prostate cancers. STAT5A and STAT6, are selectively activated when the heart is subjected to ischemic injury, whereas activation of STAT3 and STAT5A is involved in myocardial hypertrophy. STAT5 and STAT3, as these two STAT molecules are required for normal breast development and involution, respectively, and may play an important role in breast carcinogenesis. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 50% Glycerol, 1mg/ml BSA, Dulbecco's PBS (Mg2+ and Ca2+ free). pH 7.3 |
| Application Summary: | Western Blot |
| Species Summary: | Mu, |
| Alternate Names: | anti-MGF antibody, anti-Signal Transducer and Activator of Transcription 5A antibody, anti-STAT 5 antibody, anti-STAT 5A antibody, anti-STAT 5B antibody, anti-STAT5 antibody, anti-STAT5A antibody, anti-STAT5B antibody, anti-Transcription factor STAT5A antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.05 % Sodium Azide |
| Phosphorylated: | Tyr694 |
| General References: | 1. Xu W <i>et al.</i> Geldanamycin, a heat shock protein 90-binding agent, disrupts Stat5 activation in IL-2-stimulated cells. <i>J Cell Physiol</i> <b>198</b>:188-96 (2004). 2. Gutiérrez-Castellanos S <i>et al.</i> Differences in BCL-X(L) expression and STAT5 phosphorylation in chronic myeloid leukaemia patients. <i>Eur J Haematol</i> <b>72</b>:231-8 (2004). 3. Gewinner C <i>et al.</i> The coactivator of transcription CREB-binding protein interacts preferentially with the glycosylated form of Stat5. <i>J Biol Chem</i> <b>279</b>:3563-72 (2004). 4. Rico-Bautista E <i>et al.</i> Downregulation of the growth hormone-induced Janus kinase 2/signal transducer and activator of transcription 5 signaling pathway requires an intact actin cytoskeleton. <i>Exp Cell Res</i> <b>294</b>:269-80 (2004). 5. Heath, C., & Cross, N.C. Critical role of STAT5 activation in transformation mediated by ZNF198-FGFR1. J. Biol. Chem. 279(8): 6666-6673, 2004. 6. Shipley, J.M., & Waxman, D.J. Down-regulation of STAT5b transcriptional activity by ligand-activated peroxisomw proliferator-activated receptor (PPAR) alpha and PPARgamma. Mol. Pharmacol. 64(2): 355-364, 2003. 7. Okutani, Y., et al. Src directly tyrosine-phosphorylates STAT5 on its activation site and is involved in erythropoetin-induced signaling pathway. Oncogene. 20(45): 6643-6650, 2001. 8. Gesbert, F & Griffin, J.D. Bcr-Abl activates transcription of the Bcl-Xgene through STAT5. Blood. 96(6): 2269-2276, 2000. |
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