| CatalogCode: | NSB252 |
| ProductName: | c-Abl Antibody |
| Product Description: | Rabbit Polyclonal anti-c-Abl [Tyr412] |
| Clonality: | Polyclonal |
| Immunogen: | The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human c-Abl 1b that contains tyrosine 412. Note: there are two widely expressed forms of c-Abl produced by alternative splicing, designated 1b (the more common form) and 1a. The corresponding phosphorylation site from 1a is tyrosine 393. |
| CrossReactivity: | Cross-reacts with Human.Expected to cross-react with Mouse (100% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.1 ml |
| Uses: | WB: Use at a concentration of 0.1 - 1.0 ug/ml. Detects a band of approximately 140 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic and Nuclear |
| Control: | Fibroblasts transfected with oncogenic ∆SH3-Abl. |
| Background: | c-Abl is a 140-150 kDa non-receptor protein tyrosine kinase whose precise functions are not known, but roles for Abl in growth factor and integrin signaling, cell cycle regulation, cytoskeletal reorganization, neurogenesis, and responses to DNA damage and oxidative stress have been suggested. c-Abl kinase activity is increased in vivo by diverse physiological stimuli including ionizing radiation, entry into S phase, integrin activation, and platelet-derived growth factor (PDGF) stimulation. c-Abl contains various protein binding domains that appear to enable it to regulate the functions of many proteins by forming complexes, most notably three isoforms of the oncogenic protein Bcr/Abl. c-Abl becomes fully activated by sequential phosphorylation of tyrosines 412 and 245. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | Preservative: 0.05% Sodium AzideConstituents: PBS (without Mg2+ and Ca2+), 1.0mg/ml BSA (IgG, protea |
| ListPrice: | 325 |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ProteinTarget: | c-Abl phospho Y412 |
| PackageSize: | 0.1 ml |
| GeneralRef: | General / background references:Cong F et al. Interaction between UV-damaged DNA binding activity proteins and the c-Abl tyrosine kinase. J Biol Chem 277:34870-8 (2002). Furstoss O et al. c-Abl is an effector of Src for growth factor-induced c-myc expression and DNA synthesis. EMBO J 21:514-24 (2002). Brasher BB & Van Etten RA c-Abl has high intrinsic tyrosine kinase activity that is stimulated by mutation of the Src homology 3 domain and by autophosphorylation at two distinct regulatory tyrosines. J Biol Chem 275:35631-7 (2000). Plattner R et al. c-Abl is activated by growth factors and Src family kinases and has a role in the cellular response to PDGF. Genes Dev 13:2400-11 (1999). |
| Phosphorylated: | Tyr412 |