| CatalogCode: | NSB238 |
| ProductName: | Elk Antibody |
| Product Description: | Rabbit Polyclonal anti-Elk [Ser383] |
| Clonality: | Polyclonal |
| Immunogen: | The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human Elk-1 that contains serine 383. |
| CrossReactivity: | Cross-reacts with human and mouse (100% homologous) Elk-1. |
| Packaging: | 0.1 ml of epitope affinity purified rabbit antisera in PBS(without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol, with 1 mg/ml BSA (IgG, protease free) as a carrier. Contians 0.05% sodium azide (Caution: sodium azide is a poisonous and hazardous substance. Handle with care and dispose of properly.) |
| Uses: | The antibody has been used for Western blotting applications.For Western blotting applications, we recommend using the antibody at a 1:1000 dilution. *Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic (probable) |
| Control: | Y-1 adrenocortical tumor cells; NIH3T3 cells +/- EGF. |
| Background: | Elk-1, a 62 kDa transcription factor, is the most abundant component of ternary complex factors that is regulated by phosphorylation, and in turn regulates gene expression in response to multiple stimuli. Elk-1 is a substrate of MAP kinase and phosphorylated by ERK2 on several sites in the carboxyl terminus, including serine 383. Phosphorylation of Elk-1 on serine 383 is critical for its transcriptional activity, including Elk-1-mediated transcription of c-Fos. Dephosphorylation of serine 383 by calcineurin downregulates Elk-1 transcriptional activity. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| ListPrice: | 325 |
| AppSummary: | WB |
| SpeciesSummary: | Hu, Mu |
| ProteinTarget: | Elk (phospho S383) |
| PackageSize: | 0.1 ml |
| GeneralRef: | General / background references:Mócsai A et al. The immunomodulatory adapter proteins DAP12 and Fc receptor gamma-chain (FcRgamma) regulate development of functional osteoclasts through the Syk tyrosine kinase. Proc Natl Acad Sci U S A 101:6158-63 (2004). Miah SM et al. Activation of Syk protein tyrosine kinase in response to osmotic stress requires interaction with p21-activated protein kinase Pak2/gamma-PAK. Mol Cell Biol 24:71-83 (2004). Kawakami Y et al. A Ras activation pathway dependent on Syk phosphorylation of protein kinase C. Proc Natl Acad Sci U S A 100:9470-5 (2003). |
| Phosphorylated: | Ser383 |