| CatalogCode: | NSB1107 |
| ProductName: | ETS1 Antibody |
| Product Description: | Rabbit Polyclonal anti-ETS1 [Ser251] |
| Clonality: | Polyclonal |
| Immunogen: | The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human ETS1 that contains serine 251. |
| CrossReactivity: | Human ETS1. Chicken, pufferfish (Tetraodon fluviatilis), frog (Xenopus laevis), rat (each 100% homologous), mouse, rabbit (each 91% homologous) have not been tested, but are expected to react. |
| Packaging: | 0.1 ml protein A purified Rabbit antisera. |
| Uses: | The antibody has been used for Western blotting. No other applications have been tested.Recommended starting dilutions:Western Blot - 1:1000 *The optimal antibody concentration should be determined empirically for each specific application. |
| Control: | Jurkat cells treated with hydrogen peroxide or A23187. |
| Background: | The proto-oncogene ETS1 (E-twenty-six-specific sequence) is the founding member of a family of transcription factors that share a highly conserved DNA-binding domain, called the ETS domain, which recognizes a nucleotide motif with a GGAA/T core sequence. The ETS genes are dispersed on two separate chromosomal loci called ets-1, which codes for a 54 kDa protein, and ets-2, which codes for a 56 kDa protein. They are responsible for the regulation of critical genes involved in cell proliferation, differentiation, lymphoid development, motility, invasion, angiogenesis, and apoptosis. ETS1 has been shown to be phosphorylated on serine 251, 257, 282, and 285 by calmodulin-dependent kinase II in vitro and following antigenic stimulation of T or B lymphocytes or treatment with calcium ionophores in vivo. Phosphorylation at these sites, which are adjacent to the DNA binding domain, has been shown to inhibit ETS1 binding to specific DNA sequences but does not affect ETS1 localization to the nucleus. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | protein A purified |
| Host_Name: | Rabbit |
| Buffer: | 2% Sucrose, PBS |
| ListPrice: | 325 |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ALTnames: | anti-Avian erythroblastosis virus E26 (v-ets) oncogene homolog-1 antibody, anti-C-ets-1 protein antibody, anti-p54 antibody, anti-v-ets erythroblastosis virus E26 oncogene homolog 1 antibody |
| ProteinTarget: | ETS1, phospho |
| PackageSize: | 0.1 ml |
| GeneralRef: | Liu, H., et al. (2004) AML1/Runx1 recruits calcineurin to regulate granulocyte macrophage colony-stimulating factor by Ets1 activation. J. Biol. Chem. 279:29398-29408. Liu, H. and Grundstrum, T. (2002) Calcium regulation of GM-CSF by calmodulin-dependent kinase II phosphorylation of Ets1. Mol. Biol. Cell. 13:4497-4507. Yordy, J.S. and R.C. Musise-Helmericks (2000) Signal transduction and the Ets family of transcription factors. Oncogene 19:6503-6513. Cowley, D.O. and B.J. Graves (2000) Phosphorylation represses Ets-1 DNA binding by reinforcing autoinhibition. Genes Dev. 14:366-376. Rabault, B. and J. Ghysdael (1994) Calcium-induced phosphorylation of ETS1 inhibits its specific DNA binding activity. J. Biol. Chem. 269:28143-28151. Fleischman, L.F., et al. (1993) c-Ets-1 protein is hyperphosphorylated during mitosis. Oncogene 8:771-780. Fisher, C.L., et al. (1991) Ligation of membrane Ig leads to calcium-mediated phosphorylation of the proto-oncogene product, Ets-1. J. Immunol. 146:1743-1749. Pognonec, P., et al., (1988) Mitogenic stimulation of thymocytes results in the calcium-dependent phosphorylation of c-ets-1 proteins. EMBO J. 7:977-983. |
| Phosphorylated: | Ser251 |
|