| Catalog Code: | NSB1080 |
| Product Type: | Primary Antibody |
| Product Name: | Vinculin Antibody |
| List Price: | 310 |
| Clonality: | Polyclonal |
| Gene ID: | 7414 |
| Host: | Rabbit |
| Isotype: | IgG |
| Product Description: | Rabbit Polyclonal anti-Vinculin [Tyr822] |
| Phosphorylated: | Tyr822 |
| Cross Reactivity: | Chicken vinculin. Human, mouse, and rat vinculin (100% homologous) have not been tested, but are expected to react. |
| Species Summary: | Ch |
| Background: | Vinculin is an ubiquitously expressed cytoskeletal protein (~130 kDa) involved in cell adhesion and cell migration. The vinculin protein consists of a globular head domain connected to an elongated tail region by a proline-rich domain. The head region con |
| Alternate Names: | anti-VIN-1 antibody |
| Immunogen: | The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human vinculin that contains tyrosine 822. The sequence is conserved in mouse, rat, and chicken. |
| Purity: | Immunogen affinity purified |
| Packaging: | 0.1 ml of epitope affinity purified rabbit antisera in PBS(without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol, with 1.0 mg/mL BSA (IgG, protease free) as a carrier. Contians 0.05% sodium azide (Caution: sodium azide is a poisonous and hazardous subst |
| Package Size: | 0.1 ml |
| Uses: | This antibody is suitable for use in Western blotting. The recommended concentration for use in Western blotting is 1:1,000. *The optimal concentration should be determined for each specific application. |
| Application Summary: | WB |
| General References: | Opaza Saez, A.M., et al. (2003) Tension development during contractile stimulation of smooth muscle requires recruitment of paxillin and vinculin to the membrane. Am. J. Physiol. Cell Physiol. (ePub ahead of print; October 22, 2003). von Wichert, G., et a |