| Catalog Code: | NSB1078 |
| Product Type: | Primary Antibody |
| Product Name: | Vinculin Antibody |
| List Price: | 310 |
| Clonality: | Polyclonal |
| Gene ID: | 7414 |
| Host: | Rabbit |
| Isotype: | IgG |
| Product Description: | Rabbit Polyclonal anti-Vinculin [Tyr1065] |
| Phosphorylated: | Tyr1065 |
| Cross Reactivity: | Chicken and human vinculin. Mouse vinculin (100% homologous) has not been tested, but is expected to react. |
| Species Summary: | Hu, Ch |
| Background: | Vinculin is an ubiquitously expressed cytoskeletal protein (~130 kDa) involved in cell adhesion and cell migration. The vinculin protein consists of a globular head domain connected to an elongated tail region by a proline-rich domain. The head region con |
| Alternate Names: | anti-VIN-1 antibody |
| Immunogen: | The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human vinculin that contains tyrosine 1065. The sequence is conserved in mouse and chicken. |
| Purity: | Immunogen affinity purified |
| Packaging: | 0.1 ml of epitope affinity purified rabbit antisera in PBS(without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol, with 1.0 mg/mL BSA (IgG, protease free) as a carrier. Contians 0.05% sodium azide (Caution: sodium azide is a poisonous and hazardous subst |
| Package Size: | 0.1 ml |
| Uses: | This antibody is suitable for use in Western blotting, following immunoprecipitation. The recommended concentration for use in Western blotting is 1:1,000. *The optimal concentration should be determined for each specific application. |
| Application Summary: | WB |
| General References: | Zhang, Z., et al. (2004) The phosphorylation of vinculin on tyrosine residues 100 and 1065, mediated by SRC kinases, affects cell spreading. Mol. Biol. Cell. 15(9):4234-4247. Subauste, M.C., et al. (2004) Vinculin modulation of paxillin-FAK interactions |
| Notes Main: | Positive Control: COS cells co-transfected with activated Src and His-tagged chicken vinculin cDNA were treated with vanadate for 24 hr. |