| CatalogCode: | NSB1074 |
| ProductName: | Vinculin Antibody |
| Product Description: | Rabbit Polyclonal anti-Vinculin [Tyr100] |
| Clonality: | Polyclonal |
| Immunogen: | The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human vinculin that contains tyrosine 100. The sequence is conserved in mouse and chicken. |
| CrossReactivity: | Chicken and human vinculin. Mouse vinculin (100% homologous) has not been tested, but is expected to react. |
| Packaging: | 0.1 ml of epitope affinity purified rabbit antisera in PBS(without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol, with 1 mg/ml BSA (IgG, protease free) as a carrier. Contians 0.05% sodium azide (Caution: sodium azide is a poisonous and hazardous substance. Handle with care and dispose of properly.) |
| Uses: | This antibody is suitable for use in Western blotting, following immunoprecipitation. The recommended concentration for use in Western blotting is 1:1,000.*The optimal concentration should be determined for each specific application. |
| Control: | See notes. |
| Background: | Vinculin is an ubiquitously expressed cytoskeletal protein (~130 kDa) involved in cell adhesion and cell migration. The vinculin protein consists of a globular head domain connected to an elongated tail region by a proline-rich domain. The head region contains binding sites for two cytoskeletal proteins, a-actinin and talin, as well as a binding site for the tail region of vinculin itself. The tail region contains binding sites for actin, the cytoskeletal protein, paxillin, and PI(4,5)P2. In the inactive state the head region of vinculin is bound to the tail region, resulting in inaccessibility of the other protein binding sites. Binding of PI(4,5)P2 releases the head-tail interaction allowing binding of other proteins to vinculin. These regulatory events play an important role in the formation, maintenance, and breakdown of focal adhesions that occur during cell adhesion and migration. In addition to these protein binding sites, the head and tail regions of vinculin have multiple potential phosphorylation sites. Phosphorylation of vinculin on tyrosine residue 100 has been shown to play a role in cell spreading; however, it does not affect the interaction between vinculin and actin or cellular localization. The mechanism by which vinculin is phosphorylated on pY100 is unclear. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| ListPrice: | 325 |
| AppSummary: | WB |
| SpeciesSummary: | Hu, Ch |
| ALTnames: | anti-VIN-1 antibody |
| ProteinTarget: | Vinculin (phospho Y100) |
| PackageSize: | 0.1 ml |
| GeneralRef: | Zhang, Z., et al. (2004) The phosphorylation of vinculin on tyrosine residues 100 and 1065, mediated by SRC kinases, affects cell spreading. Mol. Biol. Cell. 15(9):4234-4247. Subauste, M.C., et al. (2004) Vinculin modulation of paxillin-FAK interactions regulates ERK to control survival and motility. J. Cell. Biol. 165(3):371-381. von Wichert, G., et al. (2003) Force-dependent integrin-cytoskeleton linkage formation requires downregulation of focal complex dynamics by Shp2. EMBO J. 22(19):5023-5035. Zaidel-Bar, R., et al. (2003) Early molecular events in the assembly of matrix adhesions at the leading edge of migrating cells. J. Cell Sci. 116(Pt 22):4605-4613. Ito, S., et al. (1983) Vinculin phosphorylation by the src kinase. Interaction of vinculin with phospholipid vesicles. J. Biol. Chem. 258(23):14626-14631. |
| Phosphorylated: | Tyr100 |
| NotesMain: | Positive Control: COS cells co-transfected with activated Src and His-tagged chicken vinculin cDNA were treated with vanadate for 24 hr. |
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