| CatalogCode: | | NSB1071 |
| ProductName: | | AS160 Antibody |
| Product Description: | | Rabbit Polyclonal anti-AS160 |
| Clonality: | | Polyclonal |
| Immunogen: | | Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated AS160. The final product is generated by affinity chromatography using an AS160-derived peptide that is phosphorylated at threonine 642. |
| CrossReactivity: | | Mouse, human, and rat AS160. |
| Packaging: | | 0.1 ml Immunogen affinity purified Rabbit antisera. |
| Uses: | | This antibody has been used in Western blotting.For Western blotting applications, we recommend using the antibody at a 1:1000 dilution. *The optimal antibody concentration should be determined empirically for each specific application. |
| Control: | | 3T3-L1 adipocytes or COS cells overexpressing AS160, treated with insulin. |
| Background: | | AS160 is a 160 kDa Akt substrate that plays a critical role in regulating insulin-stimulated exocytosis of glucose transporter GLUT4. AS160 contains a Rab GTPase-activating protein domain (GAP), suggesting its potential role in membrane trafficking. Insulin-stimulated phosphorylation of AS160 on threonine 642 is critical for mediating GLUT4 translocation in fat and muscle cells, via inactivation of Rab GAP function. Phosphorylation of threonine 642 is also important in mediating AS160 redistribution from low density microsomes to the cytosol in adipocytes. |
| Storage: | | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | | Immunogen affinity purified |
| Host_Name: | | Rabbit |
| Buffer: | | 1% BSA, PBS, pH 7.4 |
| ListPrice: | | 325 |
| NovusRef: | | Kumar, A., et al. Muscle-Specific Deletion of Rictor Impairs Insulin-Stimulated Glucose Transport and Enhances Basal Glycogen Synthase Activity. Mol. Cell. Biol. 28(1): 61-70, 2008. |
| AppSummary: | | WB |
| SpeciesSummary: | | Hu, Mu, Rt |
| ALTnames: | | anti-TBC1D4 antibody, anti-AOS 1 antibody, anti-AOS1 antibody, anti-HSPC140 antibody, anti-SAE 1 antibody, anti-Sentrin/SUMO Activating Protein AOS1 antibody, anti-SUA 1 antibody, anti-SUA1 antibody, anti-SUMO 1 Activating Enzyme Subunit 1 antibody, anti-Ubiquitin-like Protein SUMO 1 Activating Enzyme antibody |
| ProteinTarget: | | AS160 Phosphospecific |
| PackageSize: | | 0.1 ml |
| GeneralRef: | | 1. Zeigerer, A. et al. (2004) Insulin stimulation of GLUT4 exocytosis, but not its inhibition of endocytosis, is dependent on RabGAP AS160. Mol. Biol. Cell. 15(10):4406-4415. 2. Sano, H. et al. (2003) Insulin-stimulated phosphorylation of a Rab GTPase-activating protein regulates GLUT4 translocation. J. Biol. Chem. 278(17):14599-14602. 3. Kane, S. et al. (2002) A method to identify serine kinase substrates. Akt phosphorylates a novel adipocyte protein with a Rab GTPase-activating protein (GAP) domain. J. Biol. Chem. 277(25):22115-22118. 4. Lavan, B.E. et al (1997) A novel 160-kDa phosphotyrosine protein in insulin-treated embryonic kidney cells is a new member of the insulin receptor substrate family. J. Biol. Chem. 272(34):21403-21407. |
| more information: | | company product webpage |