| CatalogCode: | | NSB1060 |
| ProductName: | | PTEN Antibody |
| Product Description: | | Rabbit Polyclonal anti-PTEN [Ser370] |
| Clonality: | | Polyclonal |
| Immunogen: | | Synthetic peptide (Human). |
| CrossReactivity: | | Cross-reacts with Mouse. Expected to cross-react with Human (100% identity with immunogen) and Rat (100% identity with immunogen) due to sequence homology. Not yet tested in other species. |
| Packaging: | | 0.1 ml Immunogen affinity purified Rabbit antisera. |
| Uses: | | WB: Detects a band of approximately 58 kDa. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | | Nuclear |
| Control: | | 3T3-L1 adipocytes; NIH-3T3 cells. |
| Background: | | PTEN functions primarily as a lipid phosphatase to regulate crucial signal transduction pathways; a key target is phosphatidylinositol 3,4,5 trisphosphate. In addition, it displays weak tyrosine phosphatase activity, which may down modulate signaling pathways that involve focal adhesion kinase or Shc. PTEN negatively regulates Akt activation by preventing its phosphorylation. PTEN therefore inhibits the PI 3 kinase/Akt signaling pathway, which is important for cell growth and survival. Mutations of PTEN occur in 60 to 80% of prostate cancers. |
| Purity: | | Immunogen affinity purified |
| Isotype: | | IgG |
| Host_Name: | | Rabbit |
| Buffer: | | Constituents: 50% Glycerol, 1mg/ml BSA, PBS (Mg<sup>2+</sup> and Ca |
| ListPrice: | | 310 |
| AppSummary: | | WB |
| SpeciesSummary: | | Mu |
| ALTnames: | | anti-Bannayan Zonana syndrome antibody; anti-BZS antibody; anti-ITGA 2 antibody; anti-MGC11227 antibody; anti-MHAM antibody; anti-MMAC1 antibody; anti-Multiple hamartoma (Cowden syndrome) antibody; anti-Mutated in Mutiple Advanced Cancers 1 antibody; anti-Phosphatase and Tensin Homolog antibody; anti-Phosphatidylinositol 345 trisphosphate 3 phosphatase antibody; anti-Platelet antigen BR antibody; anti-PTEN1 antibody; anti-TEP1 antibody; anti-VLA 2 Receptor Alpha Subunit antibody |
| ProteinTarget: | | PTEN (phospho S370) |
| PackageSize: | | 0.1 ml |
| GeneralRef: | | <b>General / background references:</b>Wan X & Helman LJ Levels of PTEN protein modulate Akt phosphorylation on serine 473, but not on threonine 308, in IGF-II-overexpressing rhabdomyosarcomas cells. <i>Oncogene</i> <b>22</b>:8205-11 (2003). <PUBMED:14603261>Torres J <i>et al.</i> Phosphorylation-regulated cleavage of the tumor suppressor PTEN by caspase-3: implications for the control of protein stability and PTEN-protein interactions. <i>J Biol Chem</i> <b>278</b>:30652-60 (2003). <PUBMED:12788938>Birle D <i>et al.</i> Negative feedback regulation of the tumor suppressor PTEN by phosphoinositide-induced serine phosphorylation. <i>J Immunol</i> <b>169</b>:286-91 (2002). <PUBMED:12077256>Mohiuddin I <i>et al.</i> Phosphatase and tensin analog gene overexpression engenders cellular death in human malignant mesothelioma cells via inhibition of AKT phosphorylation. <i>Ann Surg Oncol</i> <b>9</b>:310-6 (2002). <PUBMED:11923140> 5. Miller, S.J., et al. Direct identification of PTEN phosphorylation sites. FEBS Letters. 528(1-3): 145-153, 2002. 6. Persad, S. et al. Tumor suppressor PTEN inhibits nuclear accumulation of beta-catenin and T cell/lymphoid enhancer factor 1-mediated transcriptional activation. J. Cell Biol. 153(6): 1161-1174, 2001. 7. Torres, J., & Pulido, R. The tumor supressor PTEN is phosphorylated by the protein kinase CK2 at its C terminus. Implications for PTEN stability to proteasome-mediated degradation. J. Biol. Chem. 276(2): 993-998, 2001. |
| Phosphorylated: | | Ser370 |
| more information: | | company product webpage |