| Catalog Code: | NB600-876 |
| Product Type: | Primary Antibody |
| Product Name: | Nestin Antibody |
| List Price: | 325 |
| Clonality: | Polyclonal |
| Gene ID: | 10763 |
| Host: | Rabbit |
| Isotype: | IgG |
| Product Description: | Rabbit Polyclonal anti-Nestin |
| Cross Reactivity: | Reacts with Mouse. Predicted to react with Human (100% identity with immunogen), Rabbit (94% identity with immunogen), Rat (88% identity with immunogen), Dog (82% identity with immunogen) and Hamster (88% identity with immunogen) due to sequence homology. |
| Species Summary: | Ca, Ha, Rt, Rb |
| Background: | Nestin is a Class VI intermediate filament expressed in the developing central nervous system (CNS) in early embryonic neuroepithelial stem cells. This protein has been widely used as a predominant marker for stem / progenitor cells, glioma cells, and tum |
| Alternate Names: | anti-ESTM 46 antibody, anti-FLJ 21841 antibody, anti-Intermediate filament protein antibody, anti-Nes antibody |
| Immunogen: | Synthetic peptide: ALETESQDSAEPSGSEE, corresponding to amino acids 1481-1497 of Human Nestin. |
| Specificity: | Detects a band at 200kDa representing Nestin in MEFs. Denaturing of WB protein lysate causes loss of Nestin immunoreactivity in WB. |
| Purity: | Immunogen affinity purified |
| Buffer: | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Packaging: | 0.1 mg of IgG purified antisera |
| Package Size: | 0.1 mg |
| Concentration: | 1 mg/ml |
| Uses: | This antibody can be used for Western blot. Detects a band of approximately 200 kDa (predicted molecular weight: 176 kDa). NB: Do not denature WB protein lysate, denaturing results in loss of Nestin specific immunoreactivity. *Optimal dilutions/concentra |
| Application Summary: | WB |