| CatalogCode: | NB600-665 |
| ProductName: | Ku70 Antibody |
| Product Description: | Rabbit Polyclonal anti-Ku70 |
| Clonality: | Polyclonal |
| Immunogen: | Full length native protein (purified) (Human). |
| Specificity: | This antibody recognises the Ku70 protein strongly in Western blotting techniques. Some weak cross-reactivity with Ku80 may be observed in certain conditions. |
| CrossReactivity: | Cross-reacts with Human, Mouse, Rat, Xenopus laevis and Hamster.Not yet tested in other species. |
| Packaging: | 0.05 ml Whole antisera Rabbit antisera. |
| Uses: | GSA: Use at an assay dependent dilution. IHC-P: Perform heat mediated antigen retrieval before commencing with IHC staining protocol. WB: Predicted molecular weight: 73 kDa. Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Nuclear |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Whole antisera |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| ListPrice: | 285 |
| AppSummary: | IP, WB, IHC-P, IHC-Fr, GS |
| ALTnames: | anti-70 kDa subunit of Ku antigen antibody, anti-ATP dependent DNA helicase II 70 kDa subunit antibody, anti-CTC box binding factor 75 kDa subunit antibody, anti-CTC75 antibody, anti-Lupus Ku autoantigen protein p70 antibody, anti-ML8 antibody, anti-Ku 70 antibody |
| ProteinTarget: | Ku70 |
| PackageSize: | 0.05 ml |
| GeneralRef: | General / background references:Blunt T et al. Defective DNA-dependent protein kinase activity is linked to V(D)J recombination and DNA repair defects associated with the murine scid mutation. Cell 80:813-23 (1995). Finnie NJ et al. DNA-dependent protein kinase activity is absent in xrs-6 cells: implications for site-specific recombination and DNA double-strand break repair. Proc Natl Acad Sci U S A 92:320-4 (1995). Kirchgessner CU et al. DNA-dependent kinase (p350) as a candidate gene for the murine SCID defect. Science 267:1178-83 (1995). Kuhn A et al. DNA-dependent protein kinase: a potent inhibitor of transcription by RNA polymerase I. Genes Dev 9:193-203 (1995). Taccioli GE et al. Ku80: product of the XRCC5 gene and its role in DNA repair and V(D)J recombination. Science 265:1442-5 (1994). Gottlieb TM & Jackson SP Protein kinases and DNA damage. Trends Biochem Sci 19:500-3 (1994). Anderson CW DNA damage and the DNA-activated protein kinase. Trends Biochem Sci 18:433-7 (1993). Gottlieb TM & Jackson SP The DNA-dependent protein kinase: requirement for DNA ends and association with Ku antigen. Cell 72:131-42 (1993). |