| CatalogCode: | NB600-629 |
| ProductName: | Angiopoietin 1 Antibody |
| Product Description: | Rabbit Polyclonal anti-Angiopoietin 1 |
| Clonality: | Polyclonal |
| Immunogen: | Peptide: CNQRRNPENGGRRYNRIQHGQ (available as ab9076)Region near the N-terminus of mouse Ang-1 (aa21-40) |
| Specificity: | This antibody is specific for mouse Angiopoietin 1 (mAng-1), and shows a band at 75kD. |
| CrossReactivity: | Cross-reacts with Mouse. Not yet tested in other species. |
| Packaging: | 0.2 ml Liquid (sterile filtered) Protein Concentration: 85 mg/ml (by Refractometry) |
| Uses: | This antibody can be used for Western Blot where there is reaction with serum in the cell supernatants, which results in strongbackground and makes it difficult to see the angiopoietins in cellsupernatants. However when precipitated (using soluble Tie2) the signals are very good and strong. Not tested in other applications.Recommended Starting Dilution:*WB: 1/500*Optimal dilutions should be determined by the end user. |
| Background: | Angiopoeitin-1 (Ang-1) and Antiopoietin-2 (Ang2) are important for development of the endothelium, by regulating tyrosine phosphorylation of the membrane receptor Tie-2/Tek. Ang-1 binding to Tie-2/Tek causes phosphorylation of the receptor. Ang-2 competes for this binding, and thus blocks receptor phosphorylation. Ang-1 has potential fibrinogen-like domain at the carboxy terminus and coiled-coil regions in the amino terminus. Ang-1 is prominently expressed in the myocardium of atrium and ventricle, mesenchymal and smooth muscle cells surrounding most blood vessels, and lung. In the adult, ANG-1 is also expressed in the heart and liver.To our knowledge, this is the first time that an Ang antibody recognises a band above 55 kD. This antibody gives a band at 75 kD which resembles the original size, because these are soluble highly glycosylated proteins, which should run higher than the calculated molecular weight of 55 kD. |
| ProductRef: | 1. Chade, A. R., Krier, J. D., Textor, S. C., Lerman, A., and Lerman, L. O. (2006) Endothelin-A Receptor Blockade Improves Renal Microvascular Architecture and Function in Experimental Hypercholesterolemia, 17, 3394-3403. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Whole antisera |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 0.02 K Phosphate, 0.15M NaCl, 0.1% Sodium Azide, pH7.2 |
| ListPrice: | 325 |
| AppSummary: | WB |
| SpeciesSummary: | Mu |
| ProteinTarget: | Angiopoietin 1 |
| PackageSize: | 0.2 ml |
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