| CatalogCode: | NB600-607 |
| ProductName: | mTOR Antibody |
| Product Description: | Rabbit Polyclonal anti-mTOR [Ser2448] |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide: KRSRTRTDpSYSAGQSVEI (Human). |
| Specificity: | Specific for phospho mTOR, giving a band at approx 250 kD. |
| CrossReactivity: | Cross-reacts with Human. Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Rabbit antisera. |
| Uses: | Optimal dilutions should be determined by the end user. |
| Localization: | Cell Membrane and Cytoplasmic |
| Background: | Mammalian target of rapamycin (mTOR) is a serine and threonine protein kinase that regulates numerous cellular functions, in particular, the initiation of protein translation. Rapamycin is a natural product macrolide that induces G1 growth arrest in yeast, Drosophila, and mammalian cells. mTOR is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, mTOR is a 245-kD protein referred to as RAFT1 with significant homology to the S. cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin-dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of mTOR to FKBP12-rapamycin correlates with the ability of these ligands to inhibit cell cycle progression. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 0.02 M Potassium Phosphate, with 0.15 M Sodium Chloride [pH 7.2] |
| ListPrice: | 325 |
| AppSummary: | ELISA, WB, IHC-P |
| SpeciesSummary: | Hu |
| ALTnames: | anti-Cell division cycle 25A isoform a antibody; anti-FK506 binding protein12-rapamycin associated protein 1 antibody; anti-FK506 binding protein12-rapamycin associated protein 2 antibody; anti-FRAP1 antibody; anti- FRAP2 antibody; anti-RAFT1 antibody; anti-RAPT1 antibody; anti-FRAP antibody |
| ProteinTarget: | mTOR |
| PackageSize: | 0.1 mg |
| GeneralRef: | Sabatini DM et al. RAFT1: a mammalian protein that binds to FKBP12 in a rapamycin-dependent fashion and is homologous to yeast TORs. Cell 78:35-43 (1994). Brown EJ et al. A mammalian protein targeted by G1-arresting rapamycin-receptor complex. Nature 369:756-8 (1994). |
| Phosphorylated: | Ser2448 |