| CatalogCode: | NB600-596 |
| ProductName: | Dab 1 Antibody |
| Product Description: | Rabbit Polyclonal anti-Dab 1 |
| Clonality: | Polyclonal |
| Immunogen: | The whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to the C-terminal region of murine Dab1 at amino acid 400-555. |
| Specificity: | This antibody does not show reactivity with Dab2. |
| CrossReactivity: | Cross-reacts with Mouse. Expected to cross-react with Human due to sequence homology. Not yet tested in other species. |
| Packaging: | 0.05 ml Whole Rabbit antisera. |
| Uses: | By Western Blot where a 80 kDa band is seen. Optimal dilutions should be determined by the end user. |
| Background: | Dab1 is a protein arising from the mouse gene dab, that is related to the Drosophila gene disabled.Mutations in the mouse dab gene may result in the 'scrambler' and 'yotari' phenotypes.Dab1 encodes a phosphoprotein that binds non-receptor tyrosine kinases and has been implicated in neuron development. Dab1 is expressed in neuronal populations exposed to reelin, as determined by in situ hybridization to embryonic mouse brain tissue. Dab1 may function as a signaling molecule that regulates positioning in the developing brain. Cloning of human Dab1 and sequence determinations suggests a 96% homology to the mouse sequence. Very low density lipoprotein receptors (VLDLR) and apolipoprotein E receptor-2 (APOER2) both bind Dab1 on their cytoplasmic tails. Dab1 expression is upregulated in knockout mice lacking both Vldlr and Apoer2 genes. In humans, Dab1 has been mapped to lp32-p312. This region shows homology of synteny with the segment of mouse chromosome 4 containing Dab1. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Whole antisera |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 0.15M Sodium Chloride, 0.02M Potassium Phosphate [pH 7] |
| ListPrice: | 285 |
| AppSummary: | WB |
| SpeciesSummary: | Mu |
| ALTnames: | anti-Disabled homolog 1 Drosophila antibody; anti-Scm antibody; anti-Scr antibody; anti-Scrambler antibody; anti-Yot antibody; anti-Yotari antibody |
| ProteinTarget: | Dab 1 |
| PackageSize: | 0.05 ml |
| GeneralRef: | D'Arcangelo G et al. Reelin is a ligand for lipoprotein receptors. Neuron 24:471-9 (1999). Trommsdorff M et al. Reeler/Disabled-like disruption of neuronal migration in knockout mice lacking the VLDL receptor and ApoE receptor 2. Cell 97:689-701 (1999). Homayouni R et al. Disabled-1 binds to the cytoplasmic domain of amyloid precursor-like protein 1. J Neurosci 19:7507-15 (1999). Alvarez-Dolado M et al. Thyroid hormone regulates reelin and dab1 expression during brain development. J Neurosci 19:6979-93 (1999). Rice DS et al. Disabled-1 acts downstream of Reelin in a signaling pathway that controls laminar organization in the mammalian brain. Development 125:3719-29 (1998). Lambert de Rouvroit C & Goffinet AM Cloning of human DAB1 and mapping to chromosome 1p31-p32. Genomics 53:246-7 (1998). Sheldon M et al. Scrambler and yotari disrupt the disabled gene and produce a reeler-like phenotype in mice. Nature 389:730-3 (1997). Howell BW et al. Neuronal position in the developing brain is regulated by mouse disabled-1. Nature 389:733-7 (1997). Ware ML et al. Aberrant splicing of a mouse disabled homolog, mdab1, in the scrambler mouse. Neuron 19:239-49 (1997). |
| NotesMain: | Genebank Accession Number NM_021080. |