| Catalog Code: | NB600-592 |
| Product Type: | Primary Antibody |
| Product Name: | NF 2 Antibody |
| List Price: | 275 |
| Clonality: | Polyclonal |
| Gene ID: | 4771 |
| Host: | Rabbit |
| Isotype: | IgG |
| Product Description: | Rabbit Polyclonal anti-NF 2 [Ser518] |
| Phosphorylated: | Ser518 |
| Cross Reactivity: | Cross-reacts with Human and Mouse. Not yet tested in other species. |
| Species Summary: | Hu, Mu |
| Background: | The recently isolated neurofibromatosis 2 tumor suppressor gene encodes a 595 amino acid protein (Merlin). The protein product Merlin, named for its relatedness to the ezrin, radixin and moesin (ERM) family of proteins, is a tumor suppressor whose absence |
| Immunogen: | Human NF2 / Merlin phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH) - TDMKRL(pS)MEIEK (aa 508-530). |
| Specificity: | This phospho specific polyclonal antibody is specific for phosphorylated Ser 518 of human NF2 (neurofibromatosis 2 gene product). Reactivity with non-phosphorylated Human NF2 (Merlin) is minimal by ELISA. Cross reactivity with NF2 (Merlin) occurs in mouse |
| Purity: | Immunogen affinity purified |
| Buffer: | 0.02 M Potassium Phosphate with 0.15 M Sodium Chloride, [pH7.2] containing 0.01% Sodium Azide |
| Packaging: | 50 micrograms purified antisera. |
| Package Size: | 0.05 mg |
| Uses: | This antibody can be used in Western blot, and capture ELISA. Has not been tested in immunohistochemistry or immunoprecipitation but is likely to be functional. Recommended Starting Dilutions*: WB: 1:1000 ELISA: 1:1000-1:5000 *Optimal dilutions should |
| Application Summary: | WB, ELISA |
| General References: | Scherer SS & Gutmann DH Expression of the neurofibromatosis 2 tumor suppressor gene product, merlin, in Schwann cells. J Neurosci Res 46:595-605 (1996). Gutmann DH et al. Expression of the neurofibromatosis 2 (NF2) gene isoforms during rat |