| CatalogCode: | NB600-585 |
| ProductName: | hnRNPC1 + C2 Antibody |
| Product Description: | Mouse Monoclonal anti-hnRNPC1 + C2 (4F4) |
| Clone: | 4F4 |
| Clonality: | Monoclonal |
| Immunogen: | RNP eluted from oligo (dT) cellulose column |
| Specificity: | This antibody detects a band at approximately 41 and 43kDa. |
| CrossReactivity: | Cross-reacts with Human, Chicken, Hamster and Monkey. Not yet tested in other species. |
| Packaging: | 0.05 ml Affinity purified Mouse ascites. |
| Uses: | In Western Blot a 41kDa (C1) and 43kDa 9C3) bands are seen.Not tested in other applications.*Optimal dilutions should be determined by the end user. |
| Localization: | Nucleus |
| Background: | RNA polymerase II transcripts in the nucleus are in complex with several proteins called heterogeneous nuclear ribonucleoproteins (hnRNPs). These proteins are important in biological activities such as transcription, pre-mRNA processing, cytoplasmic mRNA translation, and turnover. hnRNPs can be isolated either by immunoprecipitation or by sucrose gradient fractionation of cell extracts. When this is performed, the hnRNPs are isolated (consisting of protein groups named A to U), and many of these protein groups consist of more than one isoform. The major steadystate proteins of the isolated hnRNP complex are the A1, A2, B1, B2, C1, and C2 with a range of molecular weight starting with 34 kDa up to 43 kDa. The hnRNP-C proteins have a single RNP motif RNA-binding domain (RBD) of 80 to 100 amino acid long. The hnRNP -C proteins preferentially bind to uridine-rich RNA sequences. Oligomerization of the protein through leucine rich regions in its C-terminal end is important for RNA binding.4 Although its physiological action is unknown, mutation of the hnRNPC gene causes a embryonic lethal phenotype. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Affinity purified |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | 0.01 M PBS [pH 7.4) |
| ListPrice: | 285 |
| AppSummary: | ELISA, IF, IP, WB |
| SpeciesSummary: | Ch, Ha, Hu, Mk |
| ALTnames: | Anti-C1 antibody; Anti-C2 antibody; Anti-Heterogeneous nuclear ribonucleoprotein C antibody; Anti-Heterogeneous nuclear ribonucleoproteins C1/C2 antibody; Anti-HNRNP antibody; Anti-hnRNP C1 / hnRNP C2 antibody; Anti-hnRNPC antibody; Anti-HNRPC antibody; Anti-MGC104306 antibody; Anti-MGC105117 antibody; Anti-MGC117353 antibody; Anti-MGC131677 antibody; Anti-Nuclear ribonucleoprotein particle C1 protein antibody; Anti-Nuclear ribonucleoprotein particle C2 protein antibody; Anti-SNRPC antibody |
| ProteinTarget: | hnRNPC1 + C2 |
| PackageSize: | 0.05 ml |
| GeneralRef: | 1. Choi, Y.D., and Dreyfuss, G., Proc. Natl. Acad. Sci. USA, 81, 7471-7475 (1984). 2. Pinol-Roma., S., et al., J. Cell Biol., 109, 2575-2587 (1989). 3. Krecic, A.M., et al., Curr. Opin. Cell Biol., 11, 2363-2371 (1999). 4. Williamson, D.J., et al., Mol. Cell. Biol., 20, 4094-4105 (2000). |
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