| Catalog Code: | NB600-583 |
| Product Type: | Primary Antibody |
| Product Name: | Heparan Sulphate Proteoglycan Antibody |
| List Price: | 295 |
| Clonality: | Monoclonal |
| Gene ID: | 1462 |
| Host: | Rat |
| Clone: | A7L6 |
| Isotype: | IgG2a |
| Product Description: | Rat Monoclonal anti-Heparan Sulphate Proteoglycan (A7L6) |
| Cross Reactivity: | Cross-reacts with Human, Mouse, Pig and Cow. Not yet tested in other species. |
| Species Summary: | Hu, Mu, Bv |
| Background: | Proteoglycans are macromolecules consisting of a variety of core proteins with covalently attached one or several polysaccharide chains of the glycosaminoglycan type (heparan sulphate, heparin, chondroitin sulphate, dermatan sulphate or keratan sulphate). |
| Immunogen: | Murine EHS laminin preparation. |
| Localization: | Basement membrane |
| Specificity: | Recognizes domain IV of the core protein of the large heparan sulphate proteoglycan or perlecan. The reactivity is independent of the galactosaminoglycan moieties. Therefore, the epitope is not sensitive to heparitinase treatment. |
| Purity: | Tissue culture supernatant |
| Buffer: | 10mM PBS [pH7.4] with 0.2%BSA containing 0.09% sodium azide |
| Packaging: | 0.1 mg |
| Package Size: | 0.5 ml |
| Uses: | This antibody can be used in Immunofluorescence and Immunohistochemistry. For staining of acid-alcohol fixed tissues requires antigen unmasking with 15,000 ul/ml of bovine testicular hyaluronidase in PBS, pH 7.4 for 30 minutes at 37 degrees celcius. Re |
| Application Summary: | IF, IHC |
| General References: | 1. Folkvord et. al., J Histochem Cytochem, 1989; 37:105-113. 2. Couchman et. al., Matrix, 1989; 9:311-321. 3. Horiguchi et. al., J Histochem Cytochem, 1989; 37:961-970. 4. Ljubimov et. al., Int J Cancer, 1992; 50:562-566. 5. Guelstein et. al., Int J Cance |