| CatalogCode: | NB600-571 |
| ProductName: | Hsp 70 Antibody |
| Product Description: | Mouse Monoclonal anti-Hsp 70 (W27) |
| Clone: | W27 |
| Clonality: | Monoclonal |
| Immunogen: | HSP70 protein from HeLa cells. |
| Specificity: | Recognizes both the constitutive (HSP73), and inducible (HSP72) forms of HSP70. |
| CrossReactivity: | Cross-reacts with Human, Cow and Monkey. Not yet tested in other species. |
| Packaging: | 0.5 ml protein A purified Mouse ascites. |
| Uses: | This antibody can be used in Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blot. By Western blot, this antibody detects a band of 73 kDa (constitutive), 72 kDa(inducible), which corresponds to the predicted molecular weight of Hsp 70. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.*Optimal dilutions should be determined by the end user. |
| Localization: | Nuclear and cytoplasmic. |
| Control: | BT474, Hela cells or Breast carcinoma |
| Background: | Heat shock proteins (HSP's) or stress response proteins (SRP's) are synthesized in variety of environmental and pathophysiological stressful conditions. Many HSP's are involved in processes such as protein denaturation-renaturation, folding-unfolding, transport-translocation, activation-inactivation, and secretion. HSP70 is found to be associated with steroid receptors,actin, p53, polyoma T antigen, nucleotides, and other unknown proteins. Also, HSP70 has been shown to be involved in protective roles against thermal stress, cytotoxic drugs, and other damaging conditions. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | protein A purified |
| Isotype: | IgG2a |
| Host_Name: | Mouse |
| Buffer: | 10mM PBS [pH7.4] with 0.2%BSA |
| ListPrice: | 295 |
| AppSummary: | FACS, IHC, IF, IP, WB |
| SpeciesSummary: | Bv, Hu, Mk |
| ALTnames: | anti-Heat shock protein 70 antibody |
| ProteinTarget: | Hsp 70 |
| PackageSize: | 0.5 ml |
| GeneralRef: | Welch WJ & Suhan JP. Cellular and biochemical events in mammalian cells during and after recovery from physiological stress. J Cell Biol 103:2035-52 (1986). Welch WJ & Feramisco JR. Rapid purification of mammalian 70,000-dalton stress proteins: affinity of the proteins for nucleotides. Mol Cell Biol 5:1229-37 (1985). |
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