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| Catalog Code: | NB600-554 |
| Product Name: | Thrombospondin Antibody |
| Product Description: | Mouse Monoclonal anti-Thrombospondin (A4.1) |
| Clone: | A4.1 |
| Clonality: | Monoclonal |
| ListPrice: | 315 |
| Concentration: | 0.2 mg/ml |
| Immunogen: | Calcium-replete, native purified human TSP from the supernatant of thrombin activated platelets. |
| Epitope: | N-terminal half of the central stalk-like region of TSP 1 which is recovered as a 50 kDa fragment after chymotryptic digestion of TSP. |
| Specificity: | It shows no cross-reaction with fibronectin, fibrinogen or Von Willebrand factor. |
| Cross Reactivity: | Cross-reacts with Human, Rat, Mouse, Cow and Chicken. Not yet tested in other species. |
| Packaging: | 0.5 ml Affinity purified Mouse ascites. |
| Uses: | This antibody can be used in Flow Cytometry, Immunofluorescnece, and Western Blot. By Western blot, this antibody detects a band of ~ 450 kDa, which corresponds to the predicted molecular weight of Thrombospondin. This antibody blocks anti-angiogenic activity of TSP 1 by inhibiting its binding to TSP Receptor / CD36. This antibody inhibits the adhesion of human melanoma G361 cells, keratinocytes, squamous carcinoma cells, and rat smooth muscle cells to TSP 1. It does not inhibit aggregation of thrombin-induced platelets. *Optimal dilutions should be determined by the end user. |
| Localization: | Cell Membrane |
| Background: | Thrombospondin is a protein from platelet a-granules. It is made up of three identical subunits bound by interchain disulfides. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Affinity purified |
| Isotype: | IgM |
| Host Name: | Mouse |
| Buffer: | 10mM PBS [pH7.4] with 0.2%BSA |
| Application Summary: | flow cytometry / FACS analysis , immunofluorescence , Western Blot 1-2ug/ml |
| Species Summary: | Bv, Ch, Hu, Mu, Rt, |
| Alternate Names: | Anti-THBS 1 antibody; Anti-THBS antibody; Anti-THBS1 antibody; Anti-Thrombospondin 1 antibody; Anti-Tsp 1 antibody; Anti-TSP antibody; Anti-Tsp1 antibody |
| Package Size: | 0.5 ml |
| Preservative: | 0.09% sodium azide |
| General References: | 1. DeFreitas MF; Yoshida CK; Frazier WA; Mendrick DL; Kypta RM; Reichardt LF. Identification of integrin alpha 3 beta 1 as a neuronal thrombospondin receptor mediating neurite outgrowth. Neuron, 1995 Aug, 15(2):333-43. 2. RayChaudhury A; Frazier WA; D'Amore PA. Comparison of normal and tumorigenic endothelial cells: differences in thrombospondin production and responses to transforming growth factor-beta. Journal of Cell Science, 1994 Jan, 107 ( Pt 1):39-46. 3. Tolsma SS; Volpert OV; Good DJ; Frazier WA; Polverini PJ; Bouck N. Peptides derived from two separate domains of the matrix protein thrombospondin-1 have anti-angiogenic activity. Journal of Cell Biology, 1993 Jul, 122(2):497-511. 4. Frazier WA. Thrombospondins. Current Opinion in Cell Biology, 1991 Oct, 3(5):792-9. 5. Prater CA; Plotkin J; Jaye D; Frazier WA. The properdin-like type I repeats of human thrombospondin contain a cell attachment site. Journal of Cell Biology, 1991 Mar, 112(5):1031-40. 6. Good DJ; Polverini PJ; Rastinejad F; Le Beau MM; Lemons RS; Frazier WA; Bouck NP. A tumor suppressor-dependent inhibitor of angiogenesis is immunologically and functionally indistinguishable from a fragment of thrombospondin. Proceedings of the National Academy of Sciences of the United States of America, 1990 Sep, 87(17):6624-8. 7. Frazier WA. Thrombospondin: a modular adhesive glycoprotein of platelets and nucleated cells. Journal of Cell Biology, 1987 Aug, 105(2):625-32. 8. Santoro SA; Frazier WA. Isolation and characterization of thrombospondin. Methods in Enzymology, 1987, 144:438-46. 9.Prater CA, et. al. J Cell Biol, 1991, 112(5):1031-40. |
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