| CatalogCode: | NB600-536 |
| ProductName: | Actin Antibody |
| Product Description: | Mouse Monoclonal anti-Actin (1A4) |
| Clone: | 1A4 |
| Clonality: | Monoclonal |
| Immunogen: | The N-terminal decapeptide of alpha-smooth muscle actin. |
| Specificity: | This antibody reacts with the alpha smooth muscle isoform of actin. It does not react with actin from fribroblasts, striated muscle and myocardium. This antibody reacts with smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, and ovary. |
| CrossReactivity: | Cross-reacts with Human, Rabbit, Rat, Pig, and Baboon. Not yet tested in other species. |
| Packaging: | 0.5 ml Tissue culture supernatant Mouse ascites. |
| Uses: | Recommened Starting Dilutions:*Immunohistochemistry (formalin fixed paraffin embedded or frozen): 1/50 - 1/75 (30 minutes at RT).Immunocytochemistry: 1/75Immunofluorescence: 1/25 -1/50 (cells) and 1/100 (vessel sections)Not tested in other applications.*Optimal dilutions / concentrations should be determined by the end user.This antibody can also be used on frozen tissue sections for IHC. |
| Localization: | Cytoplasmic |
| Control: | Leiomyoma |
| Background: | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Tissue culture supernatant |
| Isotype: | IgG2a kappa |
| Host_Name: | Mouse |
| Buffer: | 20 mM tris-borate, 150 mM sodium chloride, |
| ListPrice: | 285 |
| AppSummary: | ELISA, IHC, IF, WB, ICC |
| SpeciesSummary: | Hu, Mk, Rt, Rb, Bb, |
| ALTnames: | anti-ActG antibody; anti-ActG1 antibody; anti-Actin antibody; anti-Gamma1 antibody; anti-DFNA20 antibody |
| ProteinTarget: | Actin |
| PackageSize: | 0.5 ml |
| GeneralRef: | 1. Skalli O <i>et al.</i> Alpha-smooth muscle actin, a differentiation marker of smooth muscle cells, is present in microfilamentous bundles of pericytes. <i>J Histochem Cytochem</i> <b>37</b>:315-21 (1989). <PUBMED:2918221> 2. Skalli O <i>et al.</i> Myofibroblasts from diverse pathologic settings are heterogeneous in their content of actin isoforms and intermediate filament proteins. <i>Lab Invest</i> <b>60</b>:275-85 (1989). <PUBMED:2644484> 3. Skalli O <i>et al.</i> A monoclonal antibody against alpha-smooth muscle actin: a new probe for smooth muscle differentiation. <i>J Cell Biol</i> <b>103</b>:2787-96 (1986). <PUBMED:3539945> |
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