| CatalogCode: | NB600-502 |
| ProductName: | GAPDH Antibody |
| Product Description: | Mouse Monoclonal anti-GAPDH (6C5) |
| Clone: | 6C5 |
| Clonality: | Monoclonal |
| Immunogen: | Rabbit muscle GAPDH. |
| Specificity: | GAPDH is a 146 kDa tetramer composed of four 30-40 kDa subunits. There is no cross-reaction with GAPDH from yeast. |
| CrossReactivity: | Cross-reacts with Human, Chicken, Dog, Fish, Mouse, Rabbit, Rat and Xenopus laevis. Does not cross-react with Cow, Goat, or s. Cerevisiae. Not yet tested in other species. |
| Packaging: | 0.1 mg protein A purified Mouse ascites. |
| Uses: | WB: Detects a band of approximately 36 kDa (predicted molecular weight: 40.2 kDa). Since GAPDH is expressed in all cells it is for example becoming the marker of choice for a loading control in Western Blotting. |
| Localization: | Cytoplasmic |
| Background: | Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. Besides its functioning as a glycolytic enzyme in cytoplasm, recent evidence suggests that mammalian GAPDH is also involved in a great number of intracellular proceses such as membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, DNA replication, and DNA repair. Recently data has implicated GAPDH in different pathologies including prostate cancer progression, programmed neuronal cell death, age-related neuronal diseases, such as Alzheimer's and Huntington's disease. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | protein A purified |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | PBS. pH 7.4 |
| ListPrice: | 245 |
| NovusRef: | 1. Kapadia, S., et al. Interference of hepatitis C virus RNA replication by short interfering RNA's. PNAS. 100(4): 2014-2018,2003. 2. Irvin, B., et al. TEL, a putative Tumor Suppressor, Induces Apoptosis and Represses Transcription of Bcl-XL. Journal of Biological Chemistry. 278(47):46378-46386, 2003. 3. Ikeda, A, Caldwell, R, Longnecker, R, Ikeda. Itchy, a Nedd4 Ubiqutin Ligase, Downregulates Latent Membrane Protein 2A Activity in B-Cell Signaling. Journal of Virology. May, 2003 Vol.77, No 9. pp.5529-5534 4. Kanadia, R.N., et al. Reversal of RNA missplicing and myotonia after muscleblind overexpression in a mouse poly(CUG) model for myotonic dystrophy. PNAS. 103:11748-11753, 2006. 5. Arthur, D.B., et al. P2Y2 receptor activates nerve growth factor/TrkA signaling to enhance neuronal differentiation. PNAS. 102:19138-19143, 2005. 6. He, H., et al. The role of microRNA genes in papillary thyroid carcinoma. PNAS 102:19075-19080, 2005. 7. Akassoglou, K., et al. Brain-specific deletion of neuropathy target esterase/swisscheese results in neurodegeneration. PNAS. 101:5075-5080, 2004. 8. Kanadia, Rahul N., et al. Reversal of RNA missplicing and myotonia after muscleblind overexpression in a mouse poly(CUG) model for myotonic dystrophy. PNAS. 103: 11748-11753, 2006 |
| AppSummary: | ELISA, WB, ICC, IHC-Fr |
| SpeciesSummary: | Bv(-), Ca, Ch, Hu, Mu, Rt, Rb, Ye(-), Gt(-), Xp |
| ALTnames: | anti-GAPDH antibody, anti-glyceraldehyde-3-phosphate dehydrogenase antibody, anti-HGNC:4141 antibody, anti-G3PD antibody, anti-GAPD antibody, anti-MGC88685 antibody |
| ProteinTarget: | GAPDH |
| PackageSize: | 0.1 mg |
| GeneralRef: | 1. Baskurt OK et al. Modulation of endothelial nitric oxide synthase expression by red blood cell aggregation. Am J Physiol Heart Circ Physiol 286:H222-9 (2004). 2. Cuezva JM et al. The bioenergetic signature of lung adenocarcinomas is a molecular marker of cancer diagnosis and prognosis. Carcinogenesis 25:1157-63 (2004). 3. Sumner CJ et al. Valproic acid increases SMN levels in spinal muscular atrophy patient cells. Ann Neurol 54:647-54 (2003). |
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