| CatalogCode: | NB600-467 |
| ProductName: | AKT1 Antibody |
| Product Description: | Rabbit Polyclonal anti-AKT1 |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide corresponding to amino acids 460-480 of the C-terminal of human, rat, mouse and chicken AKT protein conjugated to KLH. |
| CrossReactivity: | Cross-reacts with Human. Expected to cross-react with Mouse, Rat and Chicken based on sequence homology. Not yet tested in other species. |
| Packaging: | 0.1 ml Whole Rabbit antisera. |
| Uses: | Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic, and nuclear after activation by integrin linked protein kinase 1 (ILK1). |
| Control: | NIH/3T3 whole cell lysate |
| Background: | AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | Whole antisera |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | 0.02 M Potassium Phoshate, 0.15 M Sodium Chloride, pH 7.2 |
| ListPrice: | 285 |
| AppSummary: | WB, IHC-P |
| SpeciesSummary: | Hu |
| ALTnames: | Anti-C AKT antibody; Anti-CAKT antibody; Anti-MGC9965 antibody; Anti-Murine thymoma viral (vakt) oncogene homolog 1 antibody; Anti-Oncogene AKT1 antibody; Anti-PKB Akt antibody; Anti-PKB alpha antibody; Anti-PKB antibody; Anti-PKBalpha antibody; Anti-PRKBA antibody; Anti-Protein Kinase B Alpha antibody; Anti-RAC Alpha antibody; Anti-RAC alpha serine/threonine protein kinase antibody; Anti-RAC antibody; Anti-RAC PK Alpha antibody; Anti-RAC Serine/Threonine Protein Kinase antibody; Anti-V AKT Murine Thymoma Viral Oncogene Homolog 1 antibody; Anti-vAKT Murine Thymoma Viral Oncogene Homolog 1 antibody; Anti-VAKT Murine Thymona Viral Oncogene 1 antibody |
| ProteinTarget: | AKT1 |
| PackageSize: | 0.1 ml |
| GeneralRef: | Lawlor, M. A. and Alessi, D.R. (2001). PKB/AKT: a key mediator of cell proliferation, survival and insulin responses. J. Cell Science 114:2903-2910. Alessi, D. R. (2001). Discovery of PDK1, one of the missing links in insulin signal transduction. Biochem. Soc. Trans. 29,1 -14. |