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| Catalog Code: | NB600-466 |
| Product Name: | Cdc2 Antibody |
| Product Description: | Rabbit Polyclonal anti-Cdc2 |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Immunogen: | Synthetic peptide (Human) conjugated to KLH. |
| Specificity: | No reaction was observed against other related cyclin dependent kinases. The antibody inhibits the activation of p34cdc2 kinase by cyclins. |
| Cross Reactivity: | Cross-reacts with Human, Mouse and Rat. Not yet tested in other species. |
| Packaging: | 0.1 ml Whole Rabbit antisera. |
| Uses: | This antibody can be used for ELISA, Immunohistochemistry, and Western Blot.Recommended Starting Dilutions*:ELISA: 1/500-1/2000IHC: 1/200-1/1000)WB: 1/500-1/1000*Optimal dilutions should be determined by the end user. |
| Background: | p34 cdc2 is a serine-threonine protein kinase of 34,000 daltons that complexes with cyclin to form maturation promoting factor (MPF). The inactive form of the protein is phosphorylated at threonine (T) and tyrosine (Y) residues. In humans the phosphor-ylation appears to be performed by p60src. The active form of the protein is dephosphorylated and it functions by phosphorylating a number of proteins. The phosphorylation activity is coupled to the entry into the M-phase of the cell. p34 cdc2 protein must be associated with a normal cyclin protein for the M-phase to be completed normally. Association with deletion mutants of cyclin halts the M-phase before it is completed |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Whole antisera |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | No buffer, contains 0.01% sodium azide |
| Application Summary: | ELISA, Immunohistochemistry-Paraffin, Western Blot, |
| Species Summary: | Hu, Mu, Rt |
| Alternate Names: | anti-CDK1 antibody, anti-DKFZp686L20222 antibody, anti-MGC111195 antibody |
| Package Size: | 0.1 ml |
| General References: | Giordano A et al. A 60 kd cdc2-associated polypeptide complexes with the E1A proteins in adenovirus-infected cells. Cell 58:981-90 (1989). Draetta G & Beach D Activation of cdc2 protein kinase during mitosis in human cells: cell cycle-dependent phosphorylation and subunit rearrangement. Cell 54:17-26 (1988). |
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