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| Catalog Code: | NB600-465 |
| Product Name: | Cdc27 Antibody |
| Product Description: | Rabbit Polyclonal anti-Cdc27 |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Concentration: | 1.95 mg/ml |
| Immunogen: | Synthetic peptide: NINDSLEIT , corresponding to Internal sequence amino acids 422-430 of Human Cdc27. |
| Cross Reactivity: | Cross-reacts with Human. Expected to cross-react with Chicken (100% identity with immunogen) due to sequence homology. Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Rabbit antisera. |
| Uses: | In Western Blot a 90kDa band is seen (predicted MW 92 kDa)Not tested in other applications.*Optimal dilutions should be determined by the end user. |
| Localization: | Nuclear |
| Background: | Human CDC27 (also called Cell division cycle protein 27 homolog, CDC27Hs and H-NUC) shares strong similarity with Saccharomyces cerevisiae protein Cdc27. This protein is a component of naphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of a cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | 0.15M Sodium chloride and 0.02mM Potassium phosphate [pH7.2] |
| Application Summary: | ELISA 1/5000 - 1/40000, immunofluorescence , Western Blot 1/500 - 1/2000 |
| Species Summary: | Hu, |
| Alternate Names: | Anti-Anaphase Promoting Complex 3 antibody, Anti-Anaphase promoting complex protein 3 antibody, Anti-Anaphase Promoting Complex Subunit 3 antibody, Anti-APC 3 antibody, Anti-APC3 antibody, Anti-Cdc 27 antibody, Anti-CDC27Hs antibody, Anti-Cell Division Cycle 27 antibody, Anti-Cell division cycle protein 27 homolog antibody, Anti-D0S1430E antibody, Anti-D17S978E antibody, Anti-H NUC antibody, Anti-HNUC antibody, Anti-Nuc2 homolog antibody |
| Package Size: | 0.1 mg |
| Preservative: | 0.01% Sodium Azide |
| General References: | Gabellini D et al. Early mitotic degradation of the homeoprotein HOXC10 is potentially linked to cell cycle progression. EMBO J 22:3715-24 (2003). Topper LM et al. The dephosphorylated form of the anaphase-promoting complex protein Cdc27/Apc3 concentrates on kinetochores and chromosome arms in mitosis. Cell Cycle 1:282-92 (2002). Wassmann K & Benezra R Mad2 transiently associates with an APC/p55Cdc complex during mitosis. Proc Natl Acad Sci U S A 95:11193-8 (1998). |
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