| CatalogCode: | NB600-225SS |
| ProductName: | WNK1 Antibody |
| Product Description: | Rabbit Polyclonal anti-WNK1 **Sample Size** |
| Clonality: | Polyclonal |
| Immunogen: | A synthetic peptide sequence within the C-teminal region of human WNK1 (within the last 350 amino acids). |
| Specificity: | The specificity of this antibody is for WNK1. |
| CrossReactivity: | Species reactivity for NB 600-225 has been determined to work in the mouse neuroblastoma x rat dorsal root ganglia, hybridoma, F-11 lysate. There is 100% identity/homology of the immunogen with the human WNK1 protein. There is 91/99% identity/homology of the immunogen with the mouse and rat proteins. |
| Packaging: | 0.025 ml Rabbit antisera. |
| Uses: | By Western blot, this antibody recognizes the short form of WNK1, ~190 kDa. The theoretical molecular weight of human WNK1 is ~251 kD and rat WNK1 is ~230 kDa. Suggested starting dilution: Western Blot: 1:1,000 (ECL) *The investigator should determine the optimal working dilution for a specific application. |
| Background: | WNK kinases play an important role in hypertension. WNK1 is a cytoplasmic protein that localizes to a segment of kidney involved in salt, potassium, and pH homeostasis, the distal nephron. |
| Storage: | Store at 4C. Do not freeze. |
| Host_Name: | Rabbit |
| ListPrice: | 100 |
| NovusRef: | 1. Wade, J.B., et al. WNK1 kinase isoform switch regulates renal potassium excretion. PNAS. 103:8558-8563, 2006. 2. Wade, James B., et al. WNK1 kinase isoform switch regulates renal potassium excretion. PNAS. 103:8558-8563, 2006 |
| AppSummary: | WB |
| ALTnames: | anti-Kinase deficient protein antibody, anti-Protein kinase lysine deficient 1 antibody, anti-Protein kinase with no lysine 1 antibody, anti-Serine/threonine protein kinase WNK1 antibody |
| PackageSize: | 0.025 ml |
| GeneralRef: | 1. Xu, B., et al. Cell Res. 15(1): 6-10 (2005) 2. Naray-Fejes-Toth, A., et al. PNAS. 101(50): 17434-17439 (2004) 3. Wilson, FH., et al. Science. 293: 1107-1112 (2001) 4. Verissimo, F., et al. Oncogene. 20: 5562-5569 (2001) 5. Moore, TM., et al. JBC. 275(6): 4311-4322 (2000) 6. Xu, B., et al. JBC. 275(22): 16795-16801 (2000) |
| NotesMain: | A shorter spliced form of WNK1 (missing ~300 amino acids at the N-terminus) is often present in kidney tissue. It has been speculated that the short form of WNK1 is present in tumor cells, as well. Note the JBC 275(6): 4311-4322 (2000) reference for the partial transcript of human WNK1 cloned from human prostate cancer tissue. |