| CatalogCode: | NB600-207 |
| ProductName: | E2F 1 Antibody |
| Product Description: | Mouse Monoclonal anti-E2F 1 (1C5) |
| Clone: | 1C5 |
| Clonality: | Monoclonal |
| Immunogen: | Fusion protein containing the region encoding the DP-1 binding site (amino acids 223-322) of E2F-1 expressed in E. coli |
| Specificity: | This antibody is specific for human E2F-1. |
| CrossReactivity: | NB 600-207 reacts with human E2F-1. Other species untested. |
| Packaging: | 0.1 mg Mouse ascites. |
| Uses: | NB 600-207 can be used for immunoblotting and immunoprecipitation.Suggested working dilutions: *Western Blot 1-5 ug/mlImmunohistochemistry NDImmunoprecipitation 1-5 ug/ml*The investigator should determine the optimal working dilution for a specific application. |
| Control: | E2F-1 transfected Soas2 cells and recombinant fusion protein. |
| Background: | Clone 1C5 recognizes E2F-1 that was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter. The original member of the E2F family of transcription factors, now consisting of five, was identified through its association with the retinoblastoma protein, which controls its activity. E2F-1 is cell cycle regulated with very low levels in early G1 then increasing levels as cells move from G1 to S with highest levels of protein at the G1/S boundary, which is consistent with its role in S-phase entry. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG1 kappa |
| Host_Name: | Mouse |
| ListPrice: | 285 |
| AppSummary: | WB, IP |
| SpeciesSummary: | Hu |
| ALTnames: | anti-E2F-1 antibody, anti-E2F1 antibody |
| ProteinTarget: | E2F 1 |
| PackageSize: | 0.1 mg |
| GeneralRef: | 1.Shan, B., Chang, C-Y., Jones, D. and Lee, W-H. (1994) The transcription factor E2F-1 mediates the autoregulation of RB gene expression. Mol. Cell. Biol. 14:299-304. 2.Shan, B., Durfee, T. and Lee, W-H. (1996) Disruption of RB/E2F-1 interaction by single point mutations in E2F-1 enhances S-phase entry and apoptosis. Proc. Natl. Acad. Sci. USA 93:679-684. 3.Shan, B., Farmer, A.A. and Lee, W-H. (1996) The molecular basis of E2F-1/DP-1-induced S-phase entry and apoptosis. Cell Growth and Diff. 7:689-697. |
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