| Catalog Code: | NB600-206 |
| Product Type: | Primary Antibody |
| Product Name: | E2F 1 Antibody |
| List Price: | 285 |
| Clonality: | Monoclonal |
| Gene ID: | 1869 |
| Host: | Mouse |
| Clone: | 2F10 |
| Isotype: | IgG1 kappa |
| Product Description: | Mouse Monoclonal anti-E2F 1 (2F10) |
| Cross Reactivity: | NB 600-206 reacts with human E2F-1. Other species not tested. |
| Species Summary: | Hu |
| Background: | Clone 2F10 recognizes E2F-1 that was identified as a cellular protein with DNA binding activity associated with the adenovirus E2 gene promoter. The original member of the E2F family of transcription factors, now consisting of five, was identified through |
| Alternate Names: | anti-E2F-1 antibody, anti-E2F1 antibody |
| Immunogen: | Fusion protein containing the region encoding the transactivation and retinoblastoma binding domain (amino acids 332-390) of E2F-1 expressed in E. coli |
| Specificity: | This antibody is specific for human E2F-1. |
| Packaging: | 0.1 mg Mouse ascites. |
| Package Size: | 0.1 mg |
| Uses: | NB 600-206 can be used for immunoblotting and immunoprecipitation. Suggested working dilutions: * Western Blot 1-5 ug/ml Immunohistochemistry ND Immunoprecipitation 1-5 ug/ml *The investigator should determine the optimal working dilution for a specific |
| Application Summary: | WB, IP |
| General References: | 1.Shan, B., Chang, C-Y., Jones, D. and Lee, W-H. (1994) The transcription factor E2F-1 mediates the autoregulation of RB gene expression. Mol. Cell. Biol. 14:299-304. 2.Shan, B., Durfee, T. and Lee, W-H. (1996) Disruption of RB/E2F-1 interaction by single |