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| Catalog Code: | NB600-201 |
| Product Name: | Protein Kinase C Antibody |
| Product Description: | Mouse Monoclonal anti-Protein Kinase C (MC5) |
| Clone: | MC5 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Immunogen: | Purified bovine brain protein kinase C |
| Cross Reactivity: | Cross-reacts with Human, Rat and Mouse. Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Mouse ascites. |
| Uses: | ELISA: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution. IF: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. RIA: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Not tested in any other applications. |
| Background: | Protein Kinase C is a family of serine/threonine protein kinases that are activated by Ca2+, diacylglycerol and membrane phospholipids (phosphatidylserine). MC5 blocks partial proteolysis of PKC by trypsin in vitro. Fab fragment inhibits phorbol-ester-induced down-regulation of PKC in a rodent gloma cell line. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG2a |
| Host Name: | Mouse |
| Buffer: | Phosphate buffered saline |
| Application Summary: | ELISA , immunofluorescence , immunoprecipitation , Western Blot , Immunocytochemistry , Immunohistochemistry-Paraffin , Immunohistochemistry-Frozen , Radioimmunoassay |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-Protein Kinase C antibody, anti-Zeta antibody |
| Package Size: | 0.1 mg |
| Notes Main: | -epitope mapping within the hinge region of PKC (residues 292-317).-recognizes alpha, beta and gamma PKC isoforms. |
| General References: | General / background references:Van der Zee EA et al. Differential distribution of protein kinase C (PKC alpha beta and PKC gamma) isoenzyme immunoreactivity in the chick brain. Brain Res 676:41-52 (1995). <PUBMED:7796177>Ferro TJ et al. Tumor necrosis factor-alpha activates pulmonary artery endothelial protein kinase C. Am J Physiol 264:L7-14 (1993). <PUBMED:8430818>Young S et al. A monoclonal antibody recognizing the site of limited proteolysis of protein kinase C. Inhibition of down-regulation in vivo. Eur J Biochem 173:247-52 (1988). <PUBMED:2451608> |
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