| CatalogCode: | NB600-1522 |
| ProductName: | Tin2 Antibody |
| Product Description: | Mouse Monoclonal anti-Tin2 (59B388) |
| Clone: | 59B388 |
| Clonality: | Monoclonal |
| Immunogen: | A synthetic peptide corresponding to amino acids 44-58 of human Tin2 was used as immunogen. |
| CrossReactivity: | Cross-reacts with Human. Expected to cross-react with Mouse (87% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.1 mg protein G purified Mouse ascites. |
| Uses: | WB: Detects a band of approximately 40 kDa. |
| Localization: | Nuclear |
| Background: | TRF1 is a negative regulator of telomere length maintenance (3). Recently two proteins, tankyrase and Tin2, which bind to mammalian TRF1, have been identified. Tankyrase is a protein with homology to ankyrins as well as to the catalytic domain of poly (adenosine diphosphate-ribose) polymerase (PARP) (3). Tankyrase localizes to telomeres by binding to the telomeric repeat binding factor 1 (TRF1) through its ankyrin repeats (2,3). Tankyrase exhibits PARP activity functioning as acceptors for adenosine diphosphate (ADP)-ribosylation. Since ADP-ribosylation of TRF1 diminishes its ability to bind to telomeric DNA, this suggests that telomere function in human cells is regulated by poly (ADP)-ribosylation. Both the cell cycle and TRF1 may regulate the subcellular localization of tankyrase. The cDNA coding for TIN2 protein was isolated as interacting partner of TRF1 from a yeast two-hybrid cDNA library screening (1). Tin2 localizes to telomeres and is essential for proper regulation of telomere length. TIN2, like TRF1, is widely and constitutively expressed, suggesting that these proteins act together to counterbalance telomere elongation by telomerase. However, endogenous TIN2, like endogenousTRF1, is not highly expressed, and therefore may be difficult to visualize by immunostaining in certain cell systems. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG |
| Host_Name: | Mouse |
| Buffer: | PBS, 0.2% gelatin, 0.05 % sodium azide. Sodium azide is highly toxic. |
| ListPrice: | 325 |
| AppSummary: | WB |
| SpeciesSummary: | Hu |
| ProteinTarget: | Tin2 |
| PackageSize: | 0.1 mg |
| GeneralRef: | General / background references:Smith S & de Lange T Cell cycle dependent localization of the telomeric PARP, tankyrase, to nuclear pore complexes and centrosomes. J Cell Sci 112 ( Pt 21):3649-56 (1999). Kim SH et al. TIN2, a new regulator of telomere length in human cells. Nat Genet 23:405-12 (1999). Smith S et al. Tankyrase, a poly(ADP-ribose) polymerase at human telomeres. Science 282:1484-7 (1998). |