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| Catalog Code: | NB600-1437 |
| Product Name: | active + pro Caspase 3 Antibody |
| Product Description: | Mouse Monoclonal anti-active + pro Caspase 3 (31A1067) |
| Clone: | 31A1067 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Concentration: | 0.5 mg/ml |
| Immunogen: | Recombinant fusion protein (6xHis tagged full-length human Caspase 3 protein expressed in bacteria). |
| Cross Reactivity: | Cross-reacts with Human. Not yet tested in other species. |
| Packaging: | 0.1 mg IgG purified Mouse ascites. |
| Uses: | WB: Use at a concentration of 2 - 3 ug/ml. Predicted molecular weight: 31 kDa. |
| Localization: | Cytoplasmic |
| Background: | Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase 3 also known as Yama, CPP32, and apopain codes for a 32-kDa protein. Caspase 3 cleaves the death substrate poly(ADP ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase 3 substrates include DNA-PK, actin, GAS2, and procaspase 6, etc. Caspase 3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17 kDa and a small subunit of 12 kDa. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | IgG purified |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Buffer: | Constituents: PBS |
| Application Summary: | Western Blot 2 - 3 ug/ml |
| Species Summary: | Hu, |
| Alternate Names: | anti-Active caspase 3 antibody, anti-Apopain antibody, anti-CASP 3 antibody, anti-CASP3 antibody, anti-Caspase 3 activated antibody, anti-Caspase 3 antibody, anti-Caspase 3 apoptosis related cysteine peptidase antibody, anti-Caspase 3 preproprotein antibody, anti-Caspase3 antibody, anti-CPP 32 antibody, anti-CPP 32B antibody, anti-CPP32 antibody, anti-CPP32B antibody, anti-Cysteine protease CPP 32 antibody, anti-Cysteine protease CPP32 antibody, anti-PARP cleavage protease antibody, anti-Pro caspase3 antibody, anti-Procaspase 3 antibody, anti-Procaspase3 antibody, anti-SCA 1 antibody, anti-SCA1 antibody, anti-SREBP cleavage activity 1 antibody, anti-Yama antibody, anti-Yama protein antibody |
| Package Size: | 0.1 mg |
| Preservative: | 0.02% Sodium Azide |
| General References: | General / background references:Cohen GM Caspases: the executioners of apoptosis. Biochem J 326 ( Pt 1):1-16 (1997). Tewari M et al. Yama/CPP32 beta, a mammalian homolog of CED-3, is a CrmA-inhibitable protease that cleaves the death substrate poly(ADP-ribose) polymerase. Cell 81:801-9 (1995). Fernandes-Alnemri T et al. CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme. J Biol Chem 269:30761-4 (1994). |
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