| CatalogCode: | NB600-1363 |
| ProductName: | HIF1alpha Antibody |
| Product Description: | Mouse Monoclonal anti-HIF1alpha (OZ12) |
| Clone: | OZ12 |
| Clonality: | Monoclonal |
| Immunogen: | Synthetic peptide, corresponding to amino acids 530-826 of Human HIF 1 alpha. |
| CrossReactivity: | Cross-reacts with Human. Not yet tested in other species. |
| Packaging: | 0.5 ml IgG purified Mouse ascites. |
| Uses: | GSA: Use at an assay dependant dilution. IF: Use at an assay dependant dilution. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic in normoxia, nuclear translocation in response to hypoxia. |
| Control: | Mammalian cells |
| Background: | Oxygen availability plays a major role in the regulation of expression of many different genes including erythropoietin, nitric oxide synthase (NOS), heme oxygenase 1 (HO-1), glucose transporters and vascular growth factors necessary for the maintenance of homeostasis in hypoxic conditions. Hypoxia-inducible factor 1 alpha (HIF1 alpha) has been identified as a bHLH-PAS family member that is instrumental in the oxygen-dependent regulation of these genes. HIF1 alpha rapidly accumulates in the nucleus upon exposure to hypoxic conditions where it heterodimerises with the aryl hydrocarbon nuclear receptor translocator ARNT (also referred to as HIF1 beta). Heterodimerisation promotes efficient DNA binding. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | IgG purified |
| Isotype: | IgG1 kappa |
| Host_Name: | Mouse |
| Buffer: | Preservative: Sodium Azide |
| ListPrice: | 295 |
| AppSummary: | IF, GS |
| SpeciesSummary: | Hu |
| ALTnames: | anti-Basic helix loop helix PAS protein MOP1 antibody, anti-ARNT interacting protein antibody, anti-HIF 1A antibody, anti-HIF 1alpha antibody, anti-HIF1 A antibody, anti-HIF1 Alpha antibody, anti-HIF1A antibody, anti-Hypoxia inducible factor 1 alpha antibody, anti-Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor antibody, anti-Member of PAS protein 1 antibody, anti-Member of PAS superfamily 1 antibody, anti-Member of the PAS Superfamily 1 antibody, anti-MOP 1 antibody, anti-MOP1 antibody, anti-PASD 8 antibody, anti-PASD8 antibody |
| ProteinTarget: | HIF1alpha [OZ12] |
| PackageSize: | 0.5 ml |
| GeneralRef: | General / background references:Huang LE et al. Erythropoietin gene regulation depends on heme-dependent oxygen sensing and assembly of interacting transcription factors. Kidney Int 51:548-52 (1997). Semenza GL et al. Structural and functional analysis of hypoxia-inducible factor 1. Kidney Int 51:553-5 (1997). Huang LE et al. Activation of hypoxia-inducible transcription factor depends primarily upon redox-sensitive stabilization of its alpha subunit. J Biol Chem 271:32253-9 (1996). Arany Z et al. An essential role for p300/CBP in the cellular response to hypoxia. Proc Natl Acad Sci U S A 93:12969-73 (1996). |
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