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| Catalog Code: | NB600-1338 |
| Product Name: | SOCS2 Antibody |
| Product Description: | Rabbit Polyclonal anti-SOCS2 |
| Clonality: | Polyclonal |
| ListPrice: | 325 |
| Concentration: | 0.2 mg/ml |
| Immunogen: | Synthetic peptide (PTRLKDYLEEYKFQV) correspondingto amino acids 184-198 of human, rat and mouse SOCS 2. |
| Cross Reactivity: | Cross-reacts with Human, Rat and Mouse. Not yet tested in other species. |
| Packaging: | 0.1 mg Immunogen affinity purified Rabbit antisera. |
| Uses: | Immunoprecipitation: use at a concentration of 3.0 - 5.0 ug / extract from 10<sup>7</sup> cells.Western Blotting: use at a concentration of 0.5 - 2.0 ug/ml.ELISA: use at a concentration of 0.1 - 1.0 ug/ml.By Western blot, this antibody detects a band of approximately 23 kDa in GH treated HEK 293 cell lysate, which corresponds to the predicted molecular weight of SOCS 2. Samples wereboiled prior to subject 10% SDS-polyacrylamide gel electrophoresis. |
| Background: | Accumulating evidence demonstrates that cytokine receptorsignaling is negatively regulated by a family of Srchomology 2 domain-containing adaptor molecules TermedSOCS (Suppressor of Cytokine Signaling). To date, there are eight members of SOCS family have beenrecognized, they are SOCS 1, 2, 3, 4, 5, 6, 7 and CIS. Structurally the SOCS proteins are composed of an Nterminalregion of variable length and amino acidcomposition, a central SH2 domain, and a previouslyunrecognized C-terminal motif that we have called the SOCS box. The SOCS proteins appear to form part of aclassical negative feed back loop that regulates cytokinesignal transduction via a STAT induced transcriptionalmechanism. Transcription of each of the SOCS genesoccurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCSfamily appear to inhibit signaling in different ways. SOCS 2, not like SOCS 1 and SOCS 3, regulates postnatalgrowth, likely through its ability to influence the GH / IGF 1axis, although they might have overlapping actions. |
| Storage: | Store at 4C. Do not freeze. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | PBS |
| Application Summary: | ELISA 0.1-1.0 ug/ml, immunoprecipitation 1-5 ug, Western Blot 0.5-2.0 ug/ml |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | anti-CIS 2 antibody, anti-CIS2 antibody, anti-Cish 2 antibody, anti-Cish2 antibody, anti-Cytokine inducible SH2 protein 2 antibody, anti-SOCS 2 antibody, anti-SSI 2 antibody, anti-SSI2 antibody, anti-STAT induced STAT inhibitor 2 antibody, anti-STATI 2 antibody, anti-STATI2 antibody, anti-Suppressor of cytokine signaling 2 antibody |
| Package Size: | 0.1 mg |
| Preservative: | 0.05% Sodium Azide |
| General References: | Krebs DL & Hilton DJ SOCS proteins: negative regulators of cytokine signaling. Stem Cells 19:378-87 (2001). Gregorieff A et al. Regulation of SOCS-1 expression by translational repression. J Biol Chem 275:21596-604 (2000). Krebs DL & Hilton DJ SOCS: physiological suppressors of cytokine signaling. J Cell Sci 113(Pt 16):2813-9 (2000). Ram PA & Waxman DJ SOCS/CIS protein inhibition of growth hormone-stimulated STAT5 signaling by multiple mechanisms. J Biol Chem 274:35553-61 (1999). Yoshimura A The CIS family: negative regulators of JAK-STAT signaling. Cytokine Growth Factor Rev 9:197-204 (1998). Hilton DJ et al. Twenty proteins containing a C-terminal SOCS box form five structural classes. Proc Natl Acad Sci U S A 95:114-9 (1998). |
| Product References: | Ransome MI & Turnley AM Analysis of neuronal subpopulations in mice over-expressing suppressor of cytokine signaling-2. Neuroscience 132:673-87 (2005). PubMed: 15837129 |
| Novus References: | 1. Inagaki, T., Lin, V., Goetz, R., et al. Inhibition of Growth Hormone Signaling by the Fasting-Induced Hormone FGF21. Cell Metabolim. July 2008;(8)77-83 |
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