| CatalogCode: | NB600-1335 |
| ProductName: | CHOP Antibody |
| Product Description: | Mouse Monoclonal anti-CHOP (9C8) |
| Clone: | 9C8 |
| Clonality: | Monoclonal |
| Immunogen: | Bacterially expressed, mouse CHOP (GADD153) fusion protein. |
| Epitope: | NB600-1335 has been shown to recognize an epitope in the N-terminal region of GADD153. |
| CrossReactivity: | Cross-reacts with Human and Mouse. Not yet tested in other species. |
| Packaging: | 0.05 mg Mouse ascites. |
| Uses: | IP: Use at an assay dependent dilution. WB: Use at a concentration of 1 ug/ml. Predicted molecular weight: 31 kDa. |
| Localization: | Nuclear |
| Control: | Primary human fibroblasts. |
| Background: | CHOP (also known as GADD153, Growth Arrest and DNA Damage Inducible Protein 153, and C/EBP Homology Protein) is a small nuclear protein that is capable of dimerizing with transcription factors C/EBP alpha and beta. Once dimerized, this complex inhibits the normal binding and function of C/EBP to classical binding sites. Inversely, the C/EBP-CHOP dimer gains binding activity to other non-classical C/EBP stress related targets. Under normal cellular conditions this protein is not expressed in detectable levels, but is highly upregulated during times of cellular/ER stress. Examples of CHOP inducing stress include: treatment with tunicamycin, nutrient starvation and reducing agents that interfere with the calcium flux across the ER membrane. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Isotype: | IgG2b |
| Host_Name: | Mouse |
| Buffer: | Constituents: 1% BSA, PBS |
| ListPrice: | 285 |
| AppSummary: | IP, WB |
| SpeciesSummary: | Hu, Mu |
| ALTnames: | anti-GADD153 antibody |
| ProteinTarget: | GADD153 |
| PackageSize: | 0.05 mg |
| GeneralRef: | General / background references:Wang XZ et al. Cloning of mammalian Ire1 reveals diversity in the ER stress responses. EMBO J 17:5708-17 (1998). Wang XZ et al. Identification of novel stress-induced genes downstream of chop. EMBO J 17:3619-30 (1998). Batchvarova N et al. Inhibition of adipogenesis by the stress-induced protein CHOP (Gadd153). EMBO J 14:4654-61 (1995). |
| NotesMain: | This product is stable for one year from date of shipment. |