| CatalogCode: | NB600-1327 |
| ProductName: | CD11b Antibody |
| Product Description: | Rat Monoclonal anti-CD11b (M1/70.15) |
| Clone: | M1/70.15 |
| Clonality: | Monoclonal |
| Specificity: | Detects the CD11b antigen (mW170 and 95kD) also known as Mac-1 antigen, found on mouse macrophages, their precursors and granulocytes. |
| CrossReactivity: | Cross-reacts with Human, Mouse and Rabbit. Not yet tested in other species. |
| Packaging: | 0.1 mg protein G purified Rat ascites. |
| Uses: | FACS: Use at a dilution of 1/200 (10ul per million cells). IHC-P: Perform enzymatic antigen retrieval before commencing with IHC staining protocol. Optimal dilutions/concentrations should be determined by the end user. |
| Background: | CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement coated particles. It is identical to CR3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. CD11b is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. The Mac1 CD11b antigen is present on macrophages, granulocytes, natural killer cells, blood monocytes. CD11b is expressed on 8% spleen cells, 44% bone marrow cells and less than 1% of thymocytes and is commonly used as a microglial marker in nervous tissue. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG2b |
| Host_Name: | Rat |
| Buffer: | 50% Glycerol, PBS |
| ListPrice: | 285 |
| AppSummary: | FACS, IHC-P |
| SpeciesSummary: | Hu, Mu, Rb |
| ALTnames: | anti-Cell Surface Glycoprotein MAC1 Alpha Subunit antibody, anti-Complement Component Receptor 3 Alpha antibody, anti-CR3A antibody, anti-ITGAM antibody, anti-MAC1A antibody, anti-MO1A antibody, anti-Neutrophil Adherence Receptor antibody |
| ProteinTarget: | CD11b |
| PackageSize: | 0.1 mg |
| GeneralRef: | This product has been used in:Whiteland JL et al. Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies. J Histochem Cytochem 43:313-20 (1995). Smet EG et al. Mab. 198: a monoclonal antibody recognizing the complement type 3 receptor (CR3) in the rabbit. Immunology 59:419-25 (1986). Lopez AF et al. Epitope diversity of monoclonal antibodies revealed by cross-species reactivity. Mol Immunol 21:371-4 (1984). Ho MK & Springer TA Mac-1 antigen: quantitative expression in macrophage populations and tissues, and immunofluorescent localization in spleen. J Immunol 128:2281-6 (1982). Kurzinger K & Springer TA Purification and structural characterization of LFA-1, a lymphocyte function-associated antigen, and Mac-1, a related macrophage differentiation antigen associated with the type three complement receptor. J Biol Chem 257:12412-8 (1982). Ault KA & Springer TA Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells. J Immunol 126:359-64 (1981). Springer T et al. Mac-1: a macrophage differentiation antigen identified by monoclonal antibody. Eur J Immunol 9:301-6 (1979). Springer T et al. Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens. Eur J Immunol 8:539-51 (1978). General / background references:Milstein C et al. Monoclonal antibodies and cell surface antigens. Ciba Found Symp :251-76 (1979). |
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