| CatalogCode: | | NB600-1280 |
| ProductName: | | Salivary Amylase Antibody |
| Product Description: | | Sheep Polyclonal anti-Salivary Amylase |
| Clonality: | | Polyclonal |
| Immunogen: | | Human Amylase (Salivary) Type XIII-A purified from human saliva and shown to be pure by SDS-PAGE. |
| Specificity: | | This product gives one arc when tested by IEP and 2D-IEP against human saliva. Identity is confirmed by double diffusion (Ouchterlony) vs human saliva and a known anti-human salivary amylase.This product will also detect pancreatic amylase. |
| CrossReactivity: | | Cross-reacts with Human, pig and fungal. Not yet tested in other species. |
| Packaging: | | 1 ml IgG purified Sheep antisera. |
| Uses: | | For western blotting: Antigen: 50% salivaAntibody: 1:5000Secondary Antibody: 1:1000 Donkey a-Sheep/Goat immunoglobulins-peroxidaseDouble diffusion: 10uL antiserum vs 5uL salivaIEP: 100uL antiserum vs 5uL saliva Results can be enhanced by washing completed agarose gels in saline, drying and finally stainingwith a protein stain such as coomassie blue. The use of 3% PEG 6000 with 1.2%agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended. |
| Background: | | Salivary Amylase is a digestive enzyme secreted by salivary glands, it consists of a single polypeptide chain with a molecular weight of 56kDa (family B) or 62kDa (family A), the difference in molecular weight being due to the presence or absence of a carbohydrate moiety. The total serum concentration of amylase is about 120g/L of which about 50% is salivary amylase. Although salivary amylase and pancreatic amylase share 95% amino acid sequence homology, they differ in molecular size, isoelectricpoint and antigenic properties. |
| Purity: | | IgG purified |
| Isotype: | | IgG |
| Host_Name: | | Sheep |
| Buffer: | | Glycine buffered saline pH7.4, 0.1% Sodium Azide, 0.1% EACA, 0.01% Benzamidine,1mM EDTA |
| ListPrice: | | 325 |
| AppSummary: | | RID, WB |
| SpeciesSummary: | | Hu, Po |
| ProteinTarget: | | Salivary Amylase |
| PackageSize: | | 1 ml |
| more information: | | company product webpage |