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| Catalog Code: | NB600-1264 |
| Product Name: | TLR3 Antibody |
| Product Description: | Mouse Monoclonal anti-TLR3 (40C1285) |
| Clone: | 40C1285 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Concentration: | 0.5 mg/ml |
| Immunogen: | This antibody was developed against KLH-conjugated synthetic peptide of human TLR3. |
| Specificity: | This antibody has tested negative by western blot in mouse intestine cell lysate. |
| Cross Reactivity: | Cross-reacts with Human. Not yet tested in other species. |
| Packaging: | 0.05 mg protein G purified Mouse ascites. |
| Uses: | FACS: Use 2-5ug for 10<sup>6</sup> Ramos cells.IP: Use 2ug for 10<sup>6</sup> transfected cells.WB: Use at a concentration of 1 - 3 ug/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 108 kDa). Not tested in other applications.Opti |
| Localization: | Type I membrane protein |
| Background: | The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1) and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Ten human homologs of TLRs (TLR1-10) have been described (3). TLR3 cDNA codes for a protein with approximate molecular weight of 120 kDa (4). TLR3 has a restricted expression pattern being expressed in dendritic cells (DC) (4-6). TLR3 mRNA expression was detected by in situ hybridization in DC and lymph nodes (6). The expression of TLR3 in a single cell type may indicate a specific role for this molecule in a restricted setting. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG1 |
| Host Name: | Mouse |
| Buffer: | PBS containing 0.2% gelatin and 0.05% sodium azide. Sodium azide is highly toxic. |
| Application Summary: | flow cytometry / FACS analysis, immunoprecipitation, Western Blot |
| Species Summary: | Hu |
| Package Size: | 0.05 mg |
| Preservative: | 0.05% Sodium Azide |
| General References: | General / background references:Muzio M et al. Toll-like receptors: a growing family of immune receptors that are differentially expressed and regulated by different leukocytes. J Leukoc Biol 67:450-6 (2000). R |
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