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| Catalog Code: | NB600-1262 |
| Product Name: | HDAC4 Antibody |
| Product Description: | Rabbit Polyclonal anti-HDAC4 |
| Clonality: | Polyclonal |
| ListPrice: | 295 |
| Concentration: | 1 mg/ml |
| Immunogen: | Synthetic peptide: DQPVELLNPARVNHM with C-terminal added lysine, conjugated to KLH, corresponding to amino acids 14-28 of Human HDAC4.The corresponding sequence is identical in mouse and differs by one amino acid in chicken. |
| Cross Reactivity: | Cross-reacts with Human, Mouse and Rat.Expected to cross-react with Chicken (93% identity with immunogen) due to sequence homology.Not yet tested in other species. |
| Packaging: | 0.05 ml Immunogen affinity purified Rabbit antisera. |
| Uses: | IF: Use at a dilution of 1/250, this is determined by indirect immunofluorescence using HEK 293T cells expressing recombinant mouse HDAC4.IP: Use at an assay dependent dilution. 10-20 ug of antibody immunoprecipitates HDAC4 from a RIPA extract of HeLa nuclei from 1 x 10<sup>7</sup> cells.WB: Use at an assay dependent dilution. Predicted molecular weight: 140 kDa. Additional weak bands may be detected using different extract preparations. Detection of HDAC4 is specifically inhibited with the immunizing peptide.A minimum working dilution of 1/1000 is determined by blotting using whole extracts of mouse NIH-3T3 cells.A minimum working dilution of 1/500 is determined by blotting using whole extracts of rat brain.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic and Nuclear |
| Background: | Chromatin is a highly specialized structure composed of tightly compacted chromosomal DNA. Gene expression within the nucleus is controlled, in part, by a host of protein complexes which continuously pack and unpack the chromosomal DNA. One of the known mechanisms of this packing and unpacking process involves the acetylation and deacetylation of the histone proteins comprising the nucleosomal core. Acetylated histone proteins confer accessibility of the DNA template to the transcriptional machinery for expression. Histone deacetylases (HDACs) are chromatin remodeling factors that deacetylate histone proteins and thus, may act as transcriptional repressors. HDACs are classified by their sequence homology to the yeast HDACs and there are currently 2 classes. Class I proteins are related to Rpd3 and members of class II resemble Hda1p. |
| Storage: | Store at 4 degrees C short term and -20 degrees C long term. |
| Purity: | Immunogen affinity purified |
| Isotype: | IgG |
| Host Name: | Rabbit |
| Buffer: | Constituents: 1% BSA, 0.01M PBS. pH 7.4 |
| Application Summary: | immunofluorescence 1/250, immunoprecipitation assay dependent 10 to 20 ug, Western Blot , |
| Species Summary: | Hu, Mu, Rt, |
| Alternate Names: | Anti-HA6116 antibody; Anti-HD 4 antibody Anti-HD4 antibody; Anti-HDAC 4 antibody; Anti-HDAC A antibody; Anti-HDACA antibody; Anti-Histone Deacetylase 4 antibody; Anti-Histone Deacetylase A antibody; Anti-KIAA0288 antibody |
| Package Size: | 0.05 ml |
| Preservative: | 15mM Sodium Azide |
| Notes Main: | Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. |
| General References: | General / background references:Fischle W et al. The emerging role of class II histone deacetylases. Biochem Cell Biol 79:337-48 (2001). Khochbin S et al. Functional significance of histone deacetylase diversity. Curr Opin Genet Dev 11:162-6 (2001). Wang AH et al. HDAC4, a human histone deacetylase related to yeast HDA1, is a transcriptional corepressor. Mol Cell Biol 19:7816-27 (1999). Grozinger CM et al. Three proteins define a class of human histone deacetylases related to yeast Hda1p. Proc Natl Acad Sci U S A 96:4868-73 (1999). |
| Product References: | 1. Geng, L., Cuneo, K. C., Fu, A., Tu, T., Atadja, P. W., and Hallahan, D. E. (2006) Histone Deacetylase (HDAC) Inhibitor LBH589 Increases Duration of {gamma}-H2AX Foci and Confines HDAC4 to the Cytoplasm in Irradiated Non-Small Cell Lung Cancer, 66, 11298-11304. |
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