| CatalogCode: | NB600-1258 |
| ProductName: | SMAD3 Antibody |
| Product Description: | Rabbit Polyclonal anti-SMAD3 |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide corresponding to internal region of human Smad3. |
| CrossReactivity: | Reacts with Human, Mouse and Rat. Not yet tested in other species. |
| Packaging: | 0.5 ml Rabbit antisera. |
| Uses: | This antibody can be used in Immunohistochemistry-FFPE and Western Blot where a 48 kDa band is seen. For IHC-P ofr 10 min at room temperature. Perform heat mediated antigen retrieval by boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min. Not yet tested in other applications.*Optimal dilutions should be determined by the end user. |
| Localization: | In the cytoplasm in the absence of ligand; migration to the nucleus when complexed with SMAD4 |
| Control: | Normal breast or breast carcinoma |
| Background: | Smad3 is a 50 kDa member of a family of proteins that act as key mediators of TGF beta superfamily signaling in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor regulated Smads, activin/TGF beta receptor regulated (Smad2 and 3) or BMP receptor regulated (Smad 1, 5, and 8); the common partner, (Smad4) that functions via its interaction to the various Smads; and the inhibitory Smads, (Smad6 and 7). Activated Smad3 oligomerizes with Smad4 upon TGF beta stimulation and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. Phosphorylation of the two TGF beta dependent serines 423 and 425 in the C terminus of Smad3 is critical for Smad3 transcriptional activity and TGF beta signaling. |
| Storage: | Store at 4C. Do not freeze. |
| Host_Name: | Rabbit |
| Buffer: | BSA with 10mM PBS [pH 7.4] |
| ListPrice: | 295 |
| AppSummary: | ChIP, WB, IHC-P |
| SpeciesSummary: | Hu, Mu, Rt |
| ALTnames: | anti-HSPC193 antibody; anti-HST17436 antibody; anti-JV152 antibody; anti-MAD (mothers against decapentaplegic Drosophila) homolog 3 antibody; anti-MAD3 antibody; anti-MADH3 antibody; anti-Mothers against DPP homolog 3 antibody; anti-SMA and MAD related protein 3 antibody; anti-SMAD3 antibody; anti-DKFZP586N0721 antibody; anti-DKFZp686J10186 antibody; |
| ProteinTarget: | Smad3 |
| PackageSize: | 0.5 ml |