| request information: |
|
| Catalog Code: | NB600-1238 |
| Product Name: | HIF-1 alpha Antibody |
| Product Description: | Mouse Monoclonal anti-HIF-1 alpha (OZ15) |
| Clone: | OZ15 |
| Clonality: | Monoclonal |
| ListPrice: | 325 |
| Concentration: | 0.2 mg/ml |
| Immunogen: | Synthetic peptide, corresponding to amino acids 530-826 of Human HIF 1 alpha. |
| Epitope: | aa 530 - 826. |
| Cross Reactivity: | Cross-reacts with Human. Not yet tested in other species. |
| Packaging: | 0.5 ml protein G purified Mouse ascites. |
| Uses: | Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic in normoxia, nuclear translocation in response to hypoxia. |
| Background: | Oxygen availability plays a major role in the regulation of expression of many different genes including erythropoietin, nitric oxide synthase (NOS), heme oxygenase 1 (HO-1), glucose transporters and vascular growth factors necessary for the maintenance of homeostasis in hypoxic conditions. Hypoxia-inducible factor 1 alpha (HIF-1 alpha) has been identified as a bHLH-PAS family member which is instrumental in the oxygen-dependent regulation of these genes. HIF-1 alpha rapidly accumulates in the nucleus upon exposure to hypoxic conditions where it heterodimerizes with the aryl hydrocarbon nuclear receptor translocator, ARNT, also referred to as HIF-1 beta. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Purity: | protein G purified |
| Isotype: | IgG1 kappa |
| Host Name: | Mouse |
| Buffer: | 0.2% BSA, 10mM PBS. pH 7.4 |
| Application Summary: | immunofluorescence , Gel Super Shift Assays 1 ug/ml |
| Species Summary: | Hu, |
| Alternate Names: | Anti-Basic helix loop helix PAS protein MOP1 antibody, Anti-ARNT interacting protein antibody, Anti-HIF 1A antibody, Anti-HIF1A antibody, Anti-Hypoxia inducible factor 1 alpha antibody, Anti-Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor antibody, Anti-Member of PAS protein 1 antibody, Anti-Member of PAS superfamily 1 antibody, Anti-Member of the PAS Superfamily 1 antibody, Anti-MOP 1 antibody, Anti-MOP1 antibody, Anti-PASD8 antibody |
| Package Size: | 0.5 ml |
| Preservative: | 0.09% Sodium Azide |
| Notes Main: | You may use COS-7 treated and untreated nuclear extracts for your positive and negative controls for hypoxic upregulation:<br> <a href="http://www.novus-biologicals.com/data_sheet.php/18616/S/pc26">NB 800-PC26, COS-7 Nuclear Extract Kit </A><br> * The mobility of HIF-1 alpha induced by desferrioxamine or cobalt chloride treatment differs from the mobility of the hypoxia-induced protein. The reason is not known. |
| General References: | General / background references:Huang LE et al. Erythropoietin gene regulation depends on heme-dependent oxygen sensing and assembly of interacting transcription factors. Kidney Int 51:548-52 (1997). Semenza GL et al. Structural and functional analysis of hypoxia-inducible factor 1. Kidney Int 51:553-5 (1997). Huang LE et al. Activation of hypoxia-inducible transcription factor depends primarily upon redox-sensitive stabilization of its alpha subunit. J Biol Chem 271:32253-9 (1996). Arany Z et al. An essential role for p300/CBP in the cellular response to hypoxia. Proc Natl Acad Sci U S A 93:12969-73 (1996). |
| Novus References: | Veschini, L., et al. Hypoxia-inducible transcription factor-1 alpha determines sensitivity of endothelial cells to the proteosome inhibitor bortezomib. Blood, Mar 2007; 109:2565 - 2570. |
order or
more information: | company product webpage |