| CatalogCode: | NB600-1220 |
| ProductName: | alpha Actinin Antibody |
| Product Description: | Mouse Monoclonal anti-alpha Actinin (0.T.02/EA-53) |
| Clone: | 0.T.02/EA-53 |
| Clonality: | Monoclonal |
| Immunogen: | Human alpha Actinin. |
| Specificity: | Recognizes and is specific for human alpha Actinin at 100kD |
| CrossReactivity: | Cross-reacts with Human, Mouse and Rabbit. Not yet tested in other species. |
| Packaging: | 0.1 mg protein A purified Mouse ascites. |
| Uses: | ICC: Use at a concentration of 15 ug/ml. IHC: Use at a concentration of 15 ug/ml. This concentration gives positive immunostaining for alpha Actinin in MRC5 cells fixed with methanol. A previous lot of antibody detected alpha Actinin in frozen sections. WB: Use at a concentration of 0.5 - 2 ug/ml. Predicted molecular weight: 103 kDa. Detects alpha Actinin in RIPA lysates from human A431 cells. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-alpha Actinin (1mg/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Is unsuitable for IP.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Background: | Alpha Actinin is an actin binding protein present in both muscle and non-muscle cells. It has a molecular weight of 100,000 daltons and forms dimers in solution. In normal skeletal muscle alpha actinin is associated with the z-discs that define muscle sarcomeres. In smooth muscle alpha actinin has been detected in dense bodies and plaques characteristic of the tissue. Immunofluorescent labeling of a large variety of cells using antibody to alpha actinin reveals extensive association of the proteins with actin containing stress fibers and in particular with their membrane bound termini. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | protein A purified |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | Preservative: 0.05% Sodium Azide Constituents: PBS |
| ListPrice: | 295 |
| AppSummary: | IHC, WB, ICC |
| SpeciesSummary: | Hu, Mu, Rb |
| ALTnames: | anti-ACTN1 antibody, anti-Alpha Actinin 1 antibody |
| ProteinTarget: | alpha Actinin [0.T.02/EA-53] |
| PackageSize: | 0.1 mg |
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