| CatalogCode: | NB600-1200 |
| ProductName: | Raf1 Antibody |
| Product Description: | Rabbit Polyclonal anti-Raf1 |
| Clonality: | Polyclonal |
| Immunogen: | Synthetic peptide derived from the middle of RAF1 protein (Human). |
| CrossReactivity: | Cross-reacts with Human, Mouse, Rat and Frog. Not yet tested in other species. |
| Packaging: | 0.25 mg protein A purified Rabbit antisera. |
| Uses: | IP: Use at 10ug/mg of protein lysate. WB: Use at a concentration of 5 - 10 ug/ml for 2hrs at RT. Predicted molecular weight: 74 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic |
| Control: | MAD109 cells. |
| Background: | The Raf family of serine/threonine specific kinases is comprised of three members (aRaf, bRaf, and cRaf) that play a critical role in regulating cell growth and differentiation, and couple growth factor receptor stimulation to nuclear transcription factors via the Ras/mitogen activated protein kinase (MAPK) pathway. cRaf kinase (also known as Raf1) is a small GTPase like kinase of 73 kDa, and is a signal transducer of multiple extracellular stimuli that is regulated by several pathways, and that once activated, phosphorylates MEK which in turn phosphorylates ERK. Raf1 is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is part of the Ras dependent signaling pathway from receptors to the nucleus. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | protein A purified |
| Isotype: | IgG |
| Host_Name: | Rabbit |
| Buffer: | Constituents: BSA, 10mM PBS. pH 7.4 |
| ListPrice: | 295 |
| AppSummary: | IP, WB |
| SpeciesSummary: | Hu, Mu, Rt, Am |
| ALTnames: | anti-cRaf1 transforming gene antibody; anti-Murine sarcoma 3611 oncogene 1 antibody; anti-Oncogene RAF1 antibody; anti-Raf proto oncogene serine threonine protein kinase antibody; anti-Sarcoma 3611 oncogene antibody; anti-vRaf1 murine leukemia viral oncogene homologue 1 antibody |
| ProteinTarget: | Raf1 |
| PackageSize: | 0.25 mg |
| GeneralRef: | General / background references:Mason CS et al. Serine and tyrosine phosphorylations cooperate in Raf-1, but not B-Raf activation. EMBO J 18:2137-48 (1999). |