| Catalog Code: | NB600-1177 |
| Product Type: | Primary Antibody |
| Product Name: | PKR Antibody |
| List Price: | 325 |
| Clonality: | Monoclonal |
| Gene ID: | 5610 |
| Host: | Rabbit |
| Clone: | E120 |
| Isotype: | IgG |
| Product Description: | Rabbit Monoclonal anti-PKR (E120) [Thr446] |
| Phosphorylated: | Thr446 |
| Cross Reactivity: | Reacts with Human and Mouse. Does not react with Rat. Not yet tested in other species. |
| Species Summary: | Hu, Mu |
| Background: | Protein kinase R (PKR) is a serine/threonine kinase that is induced by interferon and activated by double-stranded RNA. Upon activation, PKR is autophosphorylated at multiple sites and phosphorylates the a-subunit of the eukaryotic initiation factor-2 eIF |
| Alternate Names: | anti-Double Stranded RNA Activated Interferon Inducible Protein Kinase antibody, anti-EIF2AK1 antibody, anti-Interferon inducible elF2 alpha kinase antibody, anti-P1/eIF2A protein kinase antibody, anti-p68 kinase antibody, anti-PRKR antibody |
| Immunogen: | A synthetic phospho-peptide corresponding to residues surrounding Thr446 of human PKR (Interferon-inducible RNA-dependent protein kinase) was used as immunogen. |
| Localization: | Cytoplasmic |
| Specificity: | The antibody only detects PKR phosphorylated on Threonine 446. |
| Buffer: | Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, and 0.05% BSA. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Package Size: | 0.1 ml |
| Uses: | This antibody can be used in Western Blot, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation. Recommended Starting Dilutions*: WB: 1:1,000 IHC: 1:50-1:100 ICC: 1:100 IP: 1:10 |
| Application Summary: | WB, IHC, ICC, IP |
| General References: | 1. Williams, B.R. PKR; a sentinel kinase for cellular stress. Oncogene 18: 6112-6120 (1999). 2. Cuddihy, A.R., et al. The double-stranded RNA activated protein kinase PKR physically associates with the tumor suppressor p53 protein and phosphorylates human |
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