| Catalog Code: | NB600-1168 |
| Product Type: | Primary Antibody |
| Product Name: | Histone H3 Antibody |
| List Price: | 295 |
| Clonality: | Monoclonal |
| Gene ID: | 3020 |
| Host: | Rat |
| Clone: | HTA28 |
| Isotype: | IgG2a |
| Product Description: | Rat Monoclonal anti-Histone H3 (HTA28) [Ser28] |
| Phosphorylated: | Ser28 |
| Cross Reactivity: | Cross-reacts with Human, Mouse, Cow and Hamster. Not yet tested in other species. |
| Species Summary: | Hu, Mu, Bv, Ha |
| Background: | In eukaryotic cells, DNA is associated with histones and other proteins to form chromatin. The cell division cycle constitutes a series of processes that have evolved to create two genetically identical daughter cells from a mother cell. One of these proc |
| Alternate Names: | anti-H3 antibody, anti-HisH3 antibody, anti-His1H3 antibody, anti-H3/a antibody, anti-H3/b antibody, anti-H3 antibody, c antibody, anti-H3/d antibody, anti-H3/f antibody, anti-H3/h antibody, anti-H3/i antibody, anti-H3/j antibody, anti-H3/k antibody, anti |
| Immunogen: | Synthetic peptide conjugated to KLH, corresponding to amino acids 23-35 of Human Histone H3. |
| Localization: | Nuclear |
| Specificity: | Does not detect the unphosphorylated epitope. It detects the phosphorylated histone molecule at the onset of mitosis (prophase, metaphase and weaker at the beginning of anaphase), but not during late anaphase. |
| Purity: | Immunogen affinity purified |
| Buffer: | 0.01M PBS [pH7.4] with 1% BSA containing 15mM Sodium Azide |
| Packaging: | 0.1 ml purified antisera. |
| Package Size: | 0.1 ml |
| Concentration: | 0.5 mg/ml |
| Uses: | This antibody can be used in Flow Cytometry, Immunocytochemistry, and Western Blot. By Western Blot a 15kDa band is seen. Recommended Starting Dilutions*: WB: 0.5-1.0 ug/ml *Optimal dilutions should be determined by the end user. |
| Application Summary: | FACS, ICC, WB |
| General References: | Zhong S et al. MAP kinases mediate UVB-induced phosphorylation of histone H3 at serine 28. J Biol Chem 276:12932-7 (2001). Wei Y et al. Phosphorylation of histone H3 is required for proper chromosome condensation and segregation. Cell 97: |
|