| CatalogCode: | NB600-1165 |
| ProductName: | Hsp 70 Antibody |
| Product Description: | Mouse Monoclonal anti-Hsp 70 (BRM-22) |
| Clone: | BRM-22 |
| Clonality: | Monoclonal |
| Immunogen: | Full length native protein (purified) (Bovine brain). |
| Specificity: | Monoclonal Anti-Heat Shock Protein 70 (HSP70) 70 reacts specifically against HSP70 in ELISA and immuno-blotting. In immunoblotting the antibody localizes both the constitutive (HSP73) and inducible (HSP72) forms of HSP70. The antibody recognizes brain HSP70 of bovine, human, rat, rabbit, chicken, and guinea pig. It also recognizes HSP70 in Drosophila cell extract, nematode and plant as well as human fibroblast cell extract. Immunofluorescent staining demonstrates a rapid and reversible accumulation of the HSP70 protein within the nucleus of heat-stressed (42 degrees C, 1 hr.) human fibroblasts. |
| CrossReactivity: | Cross-reacts with Human, Rat, Chicken, Cow, Drosophila melanogaster, Guinea Pig, Hamster, Plants, Rabbit and C. elegans. Not yet tested in other species. |
| Packaging: | 0.1 ml Ascites Mouse ascites. |
| Uses: | Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Cytoplasmic and Nuclear |
| Background: | Heat shock proteins are produced by prokaryotic and eukaryotic cells, and are among the most conserved molecules in phylogeny. Eukaryotic cells contain an HSP70 multigene that differ in their intracellular location and regulation. Members of the HSP70 family play a major role in the folding, unfolding and translocation of polypeptides as well as in the assembly and disassembly of oligomeric protein complexes, as well as in the immune response. HSP72 was found to increase dramatically in the brains of Alzheimer?s disease patients, and was localized exclusively in neuritic plaques and neurofibrillary tangles. HSP70 concentrates in nuclei during heat shock and returns to the cytoplasm when the shock is removed. |
| Storage: | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze thaw cycles. |
| Purity: | Ascites |
| Isotype: | IgG1 |
| Host_Name: | Mouse |
| Buffer: | 0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.01% Thimerisol, 15mg/ml BSA |
| ListPrice: | 295 |
| AppSummary: | ELISA, IF, WB, IHC-Fr |
| SpeciesSummary: | Bv, Ch, Dr, Ha, Hu, Rt, Rb, Ce, Gp, Pl |
| ALTnames: | Anti-DAQB 147D11.1 001 antibody; Anti-Heat shock 70 kDa protein 1 antibody; Anti-heat shock 70kDa protein 1A antibody; Anti-Heat shock 70kDa protein 1B antibody; Anti-heat shock protein 70 antibody; Anti-HSP70 1 antibody; Anti-HSP70 2 antibody; Anti-HSP70.1 antibody; Anti-HSP72 antibody; Anti-HSPA1 antibody; Anti-HSPA1A antibody; Anti-HSPA1B antibody; Anti-XXbac BCX40G17.3 001 antibody |
| ProteinTarget: | Hsp 70 |
| PackageSize: | 0.1 ml |
| GeneralRef: | This product has been used in:Wimmer B et al. The glyoxysomal and plastid molecular chaperones (70-kDa heat shock protein) of watermelon cotyledons are encoded by a single gene. Proc Natl Acad Sci U S A 94:13624-9 (1997). Velez-Granell CS et al. Molecular chaperones in pancreatic tissue: the presence of cpn10, cpn60 and hsp70 in distinct compartments along the secretory pathway of the acinar cells. J Cell Sci 107 (Pt 3):539-49 (1994). Marchesi VT & Ngo N In vitro assembly of multiprotein complexes containing alpha, beta, and gamma tubulin, heat shock protein HSP70, and elongation factor 1 alpha. Proc Natl Acad Sci U S A 90:3028-32 (1993). General / background references:Hamos JE et al. Expression of heat shock proteins in Alzheimer's disease. Neurology 41:345-50 (1991). Kaufmann SH Heat shock proteins and the immune response. Immunol Today 11:129-36 (1990). Omar R et al. Immunocytochemical detection of the 70-kd heat shock protein in alcoholic liver disease. Arch Pathol Lab Med 114:589-92 (1990). Mizzen LA et al. Identification, characterization, and purification of two mammalian stress proteins present in mitochondria, grp 75, a member of the hsp 70 family and hsp 58, a homolog of the bacterial groEL protein. J Biol Chem 264:20664-75 (1989). Reading DS et al. Characterization of the yeast HSP60 gene coding for a mitochondrial assembly factor. Nature 337:655-9 (1989). Reading DS et al. Characterization of the yeast HSP60 gene coding for a mitochondrial assembly factor. Nature 337:655-9 (1989). Lindquist S & Craig EA The heat-shock proteins. Annu Rev Genet 22:631-77 (1988). Pelham HR Speculations on the functions of the major heat shock and glucose-regulated proteins. Cell 46:959-61 (1986). Velazquez JM & Lindquist S hsp70: nuclear concentration during environmental stress and cytoplasmic storage during recovery. Cell 36:655-62 (1984). |
| NotesMain: | Monoclonal Anti-Heat Shock Protein 70 (mouse IgG1 isotype) is derived from the BRM-22 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified bovine brain HSP70. |
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