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| Catalog Code: | NB600-1164 |
| Product Name: | PDI Antibody |
| Product Description: | Mouse Monoclonal anti-PDI (RL77) |
| Clone: | RL77 |
| Clonality: | Monoclonal |
| ListPrice: | 295 |
| Concentration: | 2.3 mg/ml |
| Immunogen: | Recombinant full length protein (Rat). |
| Specificity: | NB 600-1164 detects protein disulphide isomerase (PDI) from human, rat and mouse tissues as well as hamster cells. |
| Cross Reactivity: | Cross-reacts with Hamster, Human, Mouse and Rat. Not yet tested in other species. |
| Packaging: | 0.1 ml Mouse ascites. |
| Uses: | IHC-P: Use at a dilution of 1/100. Antigen retrieval is not essential but may optimise staining.IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000. Detects a band of approximately 59 kDa representing PDI from rat liver extract or a slightly higher protein at 61 kDa representing PDI from human liver extract.Immunohistochemical staining of PDI in rat intestine with ab5484 yields a pattern consistent with cytoplasmic staining. In immunoprecipitation procedures ab5484 has been shown to inhibit the activity of PDI in vitro. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
| Localization: | Endoplasmic reticulum |
| Background: | The three dimensional structure of many extracellular proteins is stabilized by the formation of disulphide bonds. Studies suggest that a microsomal enzyme known as Protein Disulphide Isomerase (PDI) is involved in disulphide-bond formation and isomerization, as well as the reduction of disulphide bonds in proteins. PDI, which catalyses disulphide interchange between thiols and protein dilsulphides, has also been referred to as thiol:protein-disulphide oxidoreductase and as glutathione:insulin transhydrogenase because of its role in reduction of disulphide bonds. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the carboxy-terminus of PDI and other soluble endoplasmic reticulum (ER) resident proteins including the 78 and 94 kDa glucose regulated proteins (GRP78 and GRP94 respectively). The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor. |
| Storage: | -20 Degrees. Avoid Freeze/Thaw Cycles |
| Purity: | Ascites |
| Isotype: | IgG2b |
| Host Name: | Mouse |
| Application Summary: | immunohistochemistry 1:100, immunoprecipitation Assay dependent, Western Blot 1:1,000 |
| Species Summary: | Ha, Hu, Mu, Rt, |
| Alternate Names: | anti-Disulphide Isomerase antibody, anti-GIT antibody, anti-Glutathione Insulin Transhydrogenase antibody, anti-Procollagen Proline 2 Oxoglutarate 4 Dioxygenase beta antibody, anti-Protein Disulphide Isomerase antibody,, anti- Thyroid Hormone Binding Protein p55 antibody |
| Package Size: | 0.1 ml |
| Preservative: | 0.05% Sodium Azide |
| General References: | General / background references:Basu S & Srivastava PK Calreticulin, a peptide-binding chaperone of the endoplasmic reticulum, elicits tumor- and peptide-specific immunity. J Exp Med 189:797-802 (1999). Kuznetsov G et al. Several endoplasmic reticulum stress proteins, including ERp72, interact with thyroglobulin during its maturation. J Biol Chem 269:22990-5 (1994). Kaetzel CS et al. Protein disulphide-isomerase from human placenta and rat liver. Purification and immunological characterization with monoclonal antibodies. Biochem J 241:39-47 (1987). |
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