| Catalog Code: | NB600-1123 |
| Product Type: | Primary Antibody |
| Product Name: | Bcl-2 Antibody |
| List Price: | 245 |
| Clonality: | Monoclonal |
| Gene ID: | 596 |
| Host: | Mouse |
| Clone: | 100/D5 |
| Isotype: | IgG1 kappa |
| Product Description: | Mouse Monoclonal anti-Bcl-2 (100/D5) |
| Cross Reactivity: | Cross-reacts with Human. Does not cross-react with Rat or Mouse.Not yet tested in other species. |
| Species Summary: | Hu, Mu(-), Rt(-) |
| Background: | Expression of bcl-2a oncoprotein has been shown to inhibit the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2a protein, whereas the normal or hyperplastic germinal centers are nega |
| Immunogen: | A Synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys), of human bcl-2 protein. |
| Localization: | Endoplasmic reticulum, Outer Mitochondrial membrane and Nuclear membrane |
| Specificity: | This antibody recognizes a protein of 25-26kDa, identified as the bcl-2a oncoprotein. It does not cross-react with bcl-x or bax protein. |
| Purity: | Tissue culture supernatant |
| Buffer: | Preservative: Proprietary, not sodium azide or thimerosalConstituents: PBS, Tissue Culture Supernata |
| Packaging: | 0.5 ml This antibody is produced in tissue culture supernatant, concentrated and then placed in to PBS pH7.3 with a protein carrier and a proprietary preservative that is not sodium azide or thimerosal |
| Package Size: | 0.5 ml |
| Concentration: | 0.2 mg/ml |
| Uses: | IHC:For an ABC system, dilute 1:25-50 and incubate for 30-60 minutes at RT. For formalin fixed paraffin embedded sections, perform heat mediated antigen retrieval before commencing with IHC. Positively charged slides for tissue adhesion should be used for |
| Application Summary: | IHC-Fr, IHC-P, IP, WB |
| General References: | This product has been used in:Alderson LM et al. Human gliomas with wild-type p53 express bcl-2. Cancer Res 55:999-1001 (1995). Hurlimann J et al. bcl-2 protein in invasive ductal breast carcinomas. Virchows Arch 426:163-8 (1995). |