| CatalogCode: | NB600-1104 |
| ProductName: | HDAC2 Antibody |
| Product Description: | Rabbit Monoclonal anti-HDAC2 (Y461) |
| Clone: | Y461 |
| Clonality: | Monoclonal |
| Immunogen: | Synthetic peptide corresponding to residues in the C terminal of human HDAC2. |
| Specificity: | NB 600-1104 recognises HDAC2 |
| CrossReactivity: | Reacts with Human, Mouse and Rat. Not yet tested in other species. |
| Packaging: | 0.1 ml Rabbit ascites. |
| Uses: | This antibody can be used in Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitaion, FACS analysis, and Western Blot. In Western Blot a band is seen at 55kDa.Recommended Starting Dilutions*:FACS: 1/60 ICC/IF: 1/250-1/500IHC-P: 1/250-1/500 IP: 1/60WB: 1/2000 Not yet tested in other applications.*Optimal dilutions should be determined by the end user. |
| Localization: | Nuclear |
| Control: | Human breast carcinoma, K562 cells, HeLa cells. |
| Background: | Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDA2 is likely to be involved in regulating chromatin structure during transcription. It has been implicated to associate with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA as a cofactor. This process is highly conserved from yeast to human. |
| Storage: | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Host_Name: | Rabbit |
| Buffer: | 50 mM Tris-glycine [pH7.4] with 0.05% BSA ,40% Glycerol, & 0.15M Sodium chloride [pH 7.4] containing |
| ListPrice: | 325 |
| AppSummary: | FACS, IHC, IF, IP, WB, ICC |
| SpeciesSummary: | Hu, Mu, Rt |
| ALTnames: | anti-HD2 antibody, anti-Histone deacetylase 2 (HD2) antibody, anti-histone deacetylase 2 antibody, anti-RPD3 antibody, anti-transcriptional regulator homolog RPD3 antibody |
| ProteinTarget: | HDAC2 |
| PackageSize: | 0.1 ml |
| GeneralRef: | Hakimi MA et al. A candidate X-linked mental retardation gene is a component of a new family of histone deacetylase-containing complexes. J Biol Chem 278:7234-9 (2003). PubMed: 12493763 Rountree MR et al. DNMT1 binds HDAC2 and a new co-repressor, DMAP1, to form a complex at replication foci. Nat Genet 25:269-77 (2000). PubMed: 10888872 |
|